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HTS111M ChemiSCREEN™ Membrane Preparation Recombinant Human 5-HT6 Serotonin Receptor

Human 5-HT6 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.

ChemiSCREEN™ Membrane Preparation Recombinant Human 5-HT6 Serotonin Receptor MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Host Cell: Chem-1 Uni Prot Number: P50406 Entrez Gene Number: NM_000871
HTS111M
200 units  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

SpeciesHost CellsProtein Target
Human Chem-15-HT6
Description
Catalogue NumberHTS111M
Trade Name
  • ChemiScreen
  • Chemicon
DescriptionChemiSCREEN™ Membrane Preparation Recombinant Human 5-HT6 Serotonin Receptor
OverviewFull-length human HTR6 cDNA encoding the 5-HT6 Serotonin Receptor
Background InformationThe neurotransmitter serotonin/5-hydroxytryptamine (5-HT) regulates a wide variety of neurological functions. A family of 13 receptors (12 GPCRs and one ion channel) mediate the effects of serotonin. The serotonin receptor 5-HT6 is a Gs coupled receptor expressed solely in the CNS, primarily in the limbic and cortical regions. 5-HT6 appears to play a role in memory and learning, obesity, psychosis, anxiety and epilepsy (Woolley et al., 2004; Fisas et al., 2006). In particular, a 5-HT6-selective agonist caused significant weight loss in a rat model of diet-induced obesity. Millipore's 5-HT6 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists of 5-HT6. The membrane preparations exhibit a Kd of 0.44 nM for [125I]-SB258585. With 0.25nM [125I]-SB258585, 5 μg/well 5-HT6 Membrane Prep typically yields greater than 6-fold signal-to-background ratio.
References
Product Information
FormatMembranes
PresentationLiquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.
Quality LevelMQ100
Applications
ApplicationHuman 5-HT6 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
Key Applications
  • Radioligand binding assay
  • GTPγS binding
Application NotesRadioligand binding assay and GTPγS binding.
Biological Information
Entrez Gene Number
Gene Symbol
  • HTR6
GPCR ClassA
Host CellsChem-1
Protein Target5-HT6
Target Sub-FamilySerotonin
UniProt Number
Incubation ConditionsRECOMMENDED ASSAY CONDITIONS: Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with the binding buffer. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.

Binding buffer: 20 mM HEPES, 3 mM MgCl2, 2 mM Ascorbic acid, pH 7.4, filtered and stored at 4°C

Radioligand: [125I]-SB258585 (Perkin Elmer #:NEX424)

Wash Buffer: same as the binding buffer.

One package contains enough membranes for at least 200 assays (units), where a unit is the amount of membrane that will yield greater than 6-fold signal:background with 125I labeled SB258585 at 0.25 nM
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Table 1. Signal:background and specific binding values obtained in a competition binding assay with varying amounts of 5-HT6 Receptor membrane prep.
Product Usage Statements
5 µg/well 10 µg/well
Signal:Background 12.8 12.3
Specific Binding (cpm) 10192 12675


SPECIFICATIONS: 1 unit = 5 µg membrane preparation
Bmax for [125I]-SB258585 binding: 6.1 pmol/mg protein
Kd for [125I]-SB258585 binding: ~0.44 nM Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain frozen at –70°C up to expiration date indicated on the label. Do not freeze and thaw.
Packaging Information
Material Size200 units
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
HTS111M 04053252320705

Documentation

ChemiSCREEN™ Membrane Preparation Recombinant Human 5-HT6 Serotonin Receptor MSDS

Title

Safety Data Sheet (SDS) 

ChemiSCREEN™ Membrane Preparation Recombinant Human 5-HT6 Serotonin Receptor Certificates of Analysis

TitleLot Number
CHEMISCREENTM MEMBRANE PREPARATION RECOMBINANT HUMAN 5-HT6 SERATONIN RECEPTOR - 2324477 2324477

References

Reference overviewPub Med ID
Chronic 5-HT6 receptor modulation by E-6837 induces hypophagia and sustained weight loss in diet-induced obese rats.
Fisas, Angels, et al.
Br. J. Pharmacol., 148: 973-83 (2006)  2005

16783408 16783408
5-ht6 receptors.
Woolley, Marie L, et al.
Current drug targets. CNS and neurological disorders, 3: 59-79 (2004)  2004

Show Abstract 		14965245 14965245

Data Sheet

Title
CHEMISCREENTM MEMBRANE PREPARATION RECOMBINANT HUMAN 5-HT6 SERATONIN RECEPTOR
CHEMISCREEN™ MEMBRANE PREPARATION HUMAN RECOMBINANT 5-HT6 SEROTONIN RECEPTOR

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