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17-661 Sigma-AldrichChIPAb+ SUZ12 - ChIP Validated Antibody and Primer Set

This ChIPAb+ SUZ12 -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

ChIPAb+ SUZ12 - ChIP Validated Antibody and Primer Set MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

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References
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Key Applications: Western Blotting, Dot Blot, Chromatin Immunoprecipitation (ChIP) Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology

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Key Spec Table

Species Reactivity	Key Applications
H, M, R, Vrt	WB, DB, ChIP

Description
Catalogue Number	17-661
Brand Family	Upstate
Trade Name	ChIPAb+ Upstate
Description	ChIPAb+ SUZ12 - ChIP Validated Antibody and Primer Set
Overview	All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ SUZ12, set includes the polycomb protein SUZ12 antibody (also known as suppressor of zeste 12 protein homolog or chromatin precipitated E2F target 9 protein), a negative control antibody (purified mouse IgG), and qPCR primers which amplify a 138 bp region upstream of human HoxA2 gene. The SUZ12 and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of SUZ12 associated chromatin.
Alternate Names	SUZ12 polycomb protein SUZ12 suppressor of zeste 12 homolog

References

Product Information
Format	Purified
Control	Includes negative control mouse IgG antibody and primers specific for human HoxA2 upstream region.
Presentation	Anti-SUZ12 (mouse monoclonal IgG). One vial containing 50 μg of protein A purified antibody in PBS containing 0.05% sodium azide. May contain 30% glycerol (see certificate of analysis). Store at -20°C. Normal Mouse IgG. Two vials containing 25 μg of purified mouse IgG in 25 μL of storage buffer containing 0.1% sodium azide. Store at -20°C. ChIP Primers, HoxA2 upstream. One vial containing 75 μL of 5 μM of each primer specific for the promoter region of human HoxA2. Store at -20°C. FOR: AGG AAA GAT TTT GGT TGG GAA G REV: AAA AAG AGG GAA AGG GAC AGA C
Quality Level	MQ100

Applications
Application	This ChIPAb+ SUZ12 -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
Key Applications	Western Blotting Dot Blot Chromatin Immunoprecipitation (ChIP)
Application Notes	Chromatin Immunoprecipitation: Representative lot data. Sonicated chromatin prepared from Ntera2 cells (3 X 10⁶ cell equivalents per IP) was subjected to chromatin immunoprecipitation using 1 µg of either a normal mouse IgG or Anti-SUZ12 antibody and the Magna ChIP G Kit (Cat. # 17-611). Successful immunoprecipitation of SUZ12 associated DNA fragments was verified by qPCR using GAPDH promoter (negative) and HoxA2 (positive) Primers (Please see figures). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated. Please refer to the EZ-Magna G ChIP™ (Cat. # 17-409) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details. Western Blot Analysis: Representative lot data. Lysates from HeLa cells were resolved by electrophoresis, transferred to PVDF and probed with anti-SUZ12 (1:1,000 dilution). Proteins were visualized using goat anti-mouse secondary antibody conjugated to HRP and chemiluminescence detection (Please see figures).

Biological Information
Immunogen	SUZ12, purified antibody is made against human SUZ12.
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	Recognizes SUZ12, Mr 95 kDa.
Isotype	IgG1κ
Species Reactivity	Human Mouse Rat Vertebrates
Species Reactivity Note	Human, mouse, rat. Not tested in other species.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_056170
Entrez Gene Summary	This zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region. [provided by RefSeq]
Gene Symbol	SUZ12 JJAZ1 ChET9
UniProt Number	Q15022
UniProt Summary	Function: Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. Subunit Structure: Component of the PRC2/EED-EZH2 complex, which includes EED, EZH2, SUZ12, RBBP4 and RBBP7 and possibly AEBP2. The minimum components required for methyltransferase activity of the PRC2/EED-EZH2 complex are EED, EZH2 and SUZ12. Component of the PRC2/EED-EZH1 complex, which includes EED, EZH1, SUZ12, RBBP4 and AEBP2. The PRC2 complex may also interact with DNMT1, DNMT3A, DNMT3B and PHF1 via the EZH2 subunit and with SIRT1 via the SUZ12 subunit. Interacts with WDR77. Interacts with histone H1. Subcellular Location: Nucleus Tissue Specificity: Overexpressed in breast and colon cancer. Developmental Stage: Expressed at low levels in quiescent cells. Expression rises at the G1/S phase transition. Induction: Expression is induced by E2F1, E2F2 and E2F3. Involvement in disease: A chromosomal aberration involving SUZ12 may be a cause of endometrial stromal tumors. Translocation t(7;17)(p15;q21) with JAZF1. The translocation generates the JAZF1-SUZ12 oncogene consisting of the N-terminus part of JAZF1 and the C-terminus part of SUZ12. It is frequently found in all cases of endometrial stromal tumors, except in endometrial stromal sarcomas, where it is rarer. Sequence similarities: Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family. Contains 1 C2H2-type zinc finger.
Molecular Weight	~95 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Chromatin Immunoprecipitation: Sonicated chromatin prepared from Ntera2 cells (3 X 10⁶ cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either a normal mouse IgG or Anti-SUZ12 antibody and the Magna ChIP™ G Kit (Cat. # 17-611). Successful immunoprecipitation of SUZ12 associated DNA fragments was verified by qPCR using Control Primers (Please see figures). Please refer to the EZ-Magna ChIP™ G (Cat. # 17-409) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Chromatin Immunoprecipitation:
Sonicated chromatin prepared from Ntera2 cells (3 X 10⁶ cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either a normal mouse IgG or Anti-SUZ12 antibody and the Magna ChIP™ G Kit (Cat. # 17-611).
Successful immunoprecipitation of SUZ12 associated DNA fragments was verified by qPCR using Control Primers (Please see figures).
Please refer to the EZ-Magna ChIP™ G (Cat. # 17-409) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Packaging Information
Material Size	25 assays
Material Package	25 assays per set. Recommended use: ~2 μg antibody per chromatin immunoprecipitation (dependent upon biological context).

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
17-661	04053252738302

Documentation

ChIPAb+ SUZ12 - ChIP Validated Antibody and Primer Set MSDS

Title
Safety Data Sheet (SDS)

ChIPAb+ SUZ12 - ChIP Validated Antibody and Primer Set Certificates of Analysis

Title	Lot Number
ChIPAb+ SUZ12 - 2015591	2015591
ChIPAb+ SUZ12 - NG1806860	NG1806860
ChIPAb+ SUZ12 - NG1900364	NG1900364
ChIPAb+ SUZ12 - NRG1664847	NRG1664847
ChIPAb+SUZ12 - 2246864	2246864
ChIPAb+ SUZ12 - 2561074	2561074
ChIPAb+ SUZ12 - 3176255	3176255
ChIPAb+ SUZ12 - 3453703	3453703
ChIPAb+ SUZ12 - 3861405	3861405
ChIPAb+ SUZ12 - 3864425	3864425

References

Reference overview	Application	Pub Med ID
Epigenetic repression of the Igk locus by STAT5-mediated recruitment of the histone methyltransferase Ezh2. Mandal, M; Powers, SE; Maienschein-Cline, M; Bartom, ET; Hamel, KM; Kee, BL; Dinner, AR; Clark, MR Nature immunology 12 1212-20 2010 Show Abstract During B lymphopoiesis, recombination of the locus encoding the immunoglobulin κ-chain complex (Igk) requires expression of the precursor to the B cell antigen receptor (pre-BCR) and escape from signaling via the interleukin 7 receptor (IL-7R). By activating the transcription factor STAT5, IL-7R signaling maintains proliferation and represses Igk germline transcription by unknown mechanisms. We demonstrate that a STAT5 tetramer bound the Igk intronic enhancer (E(κi)), which led to recruitment of the histone methyltransferase Ezh2. Ezh2 marked trimethylation of histone H3 at Lys27 (H3K27me3) throughout the κ-chain joining region (J(κ)) to the κ-chain constant region (C(κ)). In the absence of Ezh2, IL-7 failed to repress Igk germline transcription. H3K27me3 modifications were lost after termination of IL-7R-STAT5 signaling, and the transcription factor E2A bound E(κi), which resulted in acquisition of H3K4me1 and acetylated histone H4 (H4Ac). Genome-wide analyses showed a STAT5 tetrameric binding motif associated with transcriptional repression. Our data demonstrate how IL-7R signaling represses Igk germline transcription and provide a general model for STAT5-mediated epigenetic transcriptional repression.	Western Blotting	22037603

Brochure

Title
Advance your Epigenetics Research
Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.

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