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219366 Cathepsin B, Bovine Spleen

Cathepsin B, Bovine Spleen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
219366
  
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Overview

Replacement Information
Description
OverviewNative cathepsin B from bovine spleen, purified by affinity chromatography. Cathepsin B, one of the most investigated lysosomal cysteine proteinases, is widely distributed in a variety of tissues. The enzyme exists in a two-polypeptide chain form consisting of an H-chain of 22 - 23 kDa and an L-chain of 5 kDa. Cathepsin B is shown to facilitate tumor invasion by dissolving extracellular barriers.

This product has been discontinued.
Catalogue Number219366
Brand Family Calbiochem®
References
ReferencesKatunuma, N., et al. 1998. Adv. Enzyme Regul. 38, 235.
Aisa, M.C., et al. 1996. Biochim. Biophys. Acta 1290, 29.
Everts, V., et al. 1996. Histochem. J. 28, 229.
Product Information
CAS number9047-22-7
Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Z-RR-<i>p</i>NA per min at 37°C, pH 6.0.
EC number3.4.22.1
FormLiquid
FormulationIn 50 mM sodium acetate buffer, 1 mM EDTA, pH 5.0.
Applications
Biological Information
Puritysingle band by non-reducing SDS-PAGE
Specific Activity≥10 units/mg protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
219366 0

Documentation

Cathepsin B, Bovine Spleen MSDS

Title

Safety Data Sheet (SDS) 

Cathepsin B, Bovine Spleen Certificates of Analysis

TitleLot Number
219366

References

Reference overview
Katunuma, N., et al. 1998. Adv. Enzyme Regul. 38, 235.
Aisa, M.C., et al. 1996. Biochim. Biophys. Acta 1290, 29.
Everts, V., et al. 1996. Histochem. J. 28, 229.

Brochure

Title
Cathepsins and Related Products Technical Bulletin
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision18-May-2010 JSW
DescriptionNative cathepsin B from bovine spleen, purified by affinity chromatography. Cathepsin B is the most investigated lysosomal cysteine proteinase distributed in a wide variety of tissues. The enzyme exists in a two-polypeptide chain form consisting of an H-chain of 22-23 kDa and an L-chain of 5 kDa.
FormLiquid
FormulationIn 50 mM sodium acetate buffer, 1 mM EDTA, pH 5.0.
CAS number9047-22-7
EC number3.4.22.1
Puritysingle band by non-reducing SDS-PAGE
Specific activity≥10 units/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Z-RR-pNA per min at 37°C, pH 6.0.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesKatunuma, N., et al. 1998. Adv. Enzyme Regul. 38, 235.
Aisa, M.C., et al. 1996. Biochim. Biophys. Acta 1290, 29.
Everts, V., et al. 1996. Histochem. J. 28, 229.