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218759 Caspase-8 Inhibitor II - Calbiochem

Caspase-8 Inhibitor II, CAS 210344-98-2, is a potent, cell-permeable, irreversible inhibitor of caspase-8 and granzyme B.

Caspase-8 Inhibitor II - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Z-IE(OMe)TD(OMe)-FMK, Granzyme B Inhibitor III

Primary Target: caspase-8
218759
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Empirical Formula
C₃₀H₄₃FN₄O₁₁

Products

Catalogue NumberPackaging Qty/Pack
218759-1MG Plastová ampulka 1 mg
218759-250UG Sklená flaša 250 μg
Description
OverviewA potent, cell-permeable, and irreversible inhibitor of caspase-8 and granzyme B. Effectively inhibits influenza virus-induced apoptosis in HeLa cells. Also inhibits granzyme B. When using with purified native or recombinant enzyme, pretreatment with an esterase is required. A 5 mM (250 µg/76 µl) solution of Z-IETD-FMK (Cat. No. 218840) in DMSO is also available.
Catalogue Number218759
Brand Family Calbiochem®
SynonymsZ-IE(OMe)TD(OMe)-FMK, Granzyme B Inhibitor III
References
ReferencesTakizawa, T., et al. 1999. Microbiol. Immunol. 43, 245.
Martin, D.A., et al. 1998. J. Biol. Chem. 273, 4345.
Sweeney, E.A., et al. 1998. FEBS Lett. 425, 61.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Product Information
ATP CompetitiveN
FormWhite to yellow powder
Hill FormulaC₃₀H₄₃FN₄O₁₁
Chemical formulaC₃₀H₄₃FN₄O₁₁
ReversibleN
Quality LevelMQ100
Applications
ApplicationCaspase-8 Inhibitor II, CAS 210344-98-2, is a potent, cell-permeable, irreversible inhibitor of caspase-8 and granzyme B.
Biological Information
Primary Targetcaspase-8
Purity≥98% by HPLC
Physicochemical Information
Cell permeableY
Peptide SequenceZ-Ile-Glu(OMe)-Thr-Asp(OMe)-CH₂F*
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Moisture Protect from moisture
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 8 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
218759-1MG 04055977218619
218759-250UG 04055977218626

Documentation

Caspase-8 Inhibitor II - Calbiochem MSDS

Title

Safety Data Sheet (SDS) 

Caspase-8 Inhibitor II - Calbiochem Certificates of Analysis

TitleLot Number
218759

References

Reference overview
Takizawa, T., et al. 1999. Microbiol. Immunol. 43, 245.
Martin, D.A., et al. 1998. J. Biol. Chem. 273, 4345.
Sweeney, E.A., et al. 1998. FEBS Lett. 425, 61.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure

Citations

Title
  • Sinead M. Miggin, et al. (2007) NF-κB activation by the Toll-IL-1 receptor domain protein MyD88 adapter-like is regulated by caspase-1. Procedings of the National Academy of Science 104, 3372-3377.
  • Kira Steigerwald, et al. (2005) The APC tumor suppressor promotes transcription-independent apoptosis in vitro. Molecular Cancer Research 3, 78-89.
  • Teresa Sanchez Alcaraz, et al. (2004) Involvement of Glutathione as a Mechanism of Indirect Protection against Spontaneous Ex Vivo Apoptosis Associated with Bovine Leukemia Virus. Journal of Virology 78, 6180-6190.
  • Ashish K. Sharma and Baerbel Rohrer. (2004) Calcium-induced Calpain Mediates Apoptosis via Caspase-3 in a Mouse Photoreceptor Cell Line. Journal of Biological Chemistry 279, 35564-35572.
  • Wayne D. Thomas, et al. (2000) TNF-Related Apoptosis-Inducing Ligand-Induced Apoptosis of Melanoma is Associated with Changes in Mitochondrial Membrane Potential and Perinuclear Clustering of Mitochondria. Journal of Immunology 165, 5612-5620.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision14-November-2023 JSW
    SynonymsZ-IE(OMe)TD(OMe)-FMK, Granzyme B Inhibitor III
    DescriptionA potent, cell-permeable, and irreversible inhibitor of caspase-8 (MACH, FLICE, Mch5). Also inhibits granzyme B.
    FormWhite to yellow powder
    Chemical formulaC₃₀H₄₃FN₄O₁₁
    Peptide SequenceZ-Ile-Glu(OMe)-Thr-Asp(OMe)-CH₂F*
    Purity≥98% by HPLC
    SolubilityDMSO (10 mM)
    Storage Protect from moisture
    -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 8 months at -20°C.
    Toxicity Standard Handling
    ReferencesTakizawa, T., et al. 1999. Microbiol. Immunol. 43, 245.
    Martin, D.A., et al. 1998. J. Biol. Chem. 273, 4345.
    Sweeney, E.A., et al. 1998. FEBS Lett. 425, 61.
    Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
    Citation
  • Sinead M. Miggin, et al. (2007) NF-κB activation by the Toll-IL-1 receptor domain protein MyD88 adapter-like is regulated by caspase-1. Procedings of the National Academy of Science 104, 3372-3377.
  • Kira Steigerwald, et al. (2005) The APC tumor suppressor promotes transcription-independent apoptosis in vitro. Molecular Cancer Research 3, 78-89.
  • Teresa Sanchez Alcaraz, et al. (2004) Involvement of Glutathione as a Mechanism of Indirect Protection against Spontaneous Ex Vivo Apoptosis Associated with Bovine Leukemia Virus. Journal of Virology 78, 6180-6190.
  • Ashish K. Sharma and Baerbel Rohrer. (2004) Calcium-induced Calpain Mediates Apoptosis via Caspase-3 in a Mouse Photoreceptor Cell Line. Journal of Biological Chemistry 279, 35564-35572.
  • Wayne D. Thomas, et al. (2000) TNF-Related Apoptosis-Inducing Ligand-Induced Apoptosis of Melanoma is Associated with Changes in Mitochondrial Membrane Potential and Perinuclear Clustering of Mitochondria. Journal of Immunology 165, 5612-5620.
    		•