Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

208718 Calpain-2, Rat, Recombinant, High Purity, E. coli

Calpain-2, Rat, Recombinant, High Purity, E. coli MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: m-Calpain

208718
  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Overview

This product has been discontinued.



Recombinant, rat calpain-2 expressed in E. coli. Ca2+-dependent heterodimeric cysteine proteinase with high Ca2+ requirement (EC50 = 350 µM Ca2+). Supplied as highly purified preparation.
Catalogue Number208718
Brand Family Calbiochem®
Synonymsm-Calpain
References
ReferencesElce, J.S., et al. 1995. Protein Eng. 8, 843.
Graham-Siegenthaler, K., et al. 1994. J. Biol. Chem. 269, 30457.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that hydrolyzes 1 pmol. Suc-leu-Leu0Val-Tyr-AMC in 1 min at 25°C using the Calpain Activity Assay Kit, Fluorometric (Cat. No. QIA120). Note: 1 caseinolytic unit = 0.8 fluorogenic units.
EC number3.4.22.17
FormLiquid
FormulationIn 100 mM NaCl, 50 mM Tris-HCl, 5 mM EDTA, 5 mM β-mercaptoethanol, 40% glycerol, pH 7.8.
Applications
Biological Information
Purity≥98% by SDS-PAGE
Specific Activity≥1500 units/mg protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/37/38-42/43

Irritating to eyes, respiratory system and skin.
May cause sensitization by inhalation and skin contact.
S PhraseS: 26-36

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Harmful
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
208718 0

Documentation

Calpain-2, Rat, Recombinant, High Purity, E. coli Certificates of Analysis

TitleLot Number
208718

References

Reference overview
Elce, J.S., et al. 1995. Protein Eng. 8, 843.
Graham-Siegenthaler, K., et al. 1994. J. Biol. Chem. 269, 30457.

Citations

Title
  • Masako Shimada, et al. (2005) The receptor for parathyroid hormone and parathyroid hormone-related peptide is hydrolyzed and its signaling properties are altered by directly binding the calpain small subunit. Endocrinology 146, 2336-2344.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision05-November-2007 JSW
    Synonymsm-Calpain
    DescriptionRecombinant, rat calpain-2 expressed in E. coli. Ca2+-dependent heterodimeric cysteine proteinase with high Ca2+ requirement (EC50 = 350 µM Ca2+). Supplied as highly purified preparation.
    FormLiquid
    FormulationIn 100 mM NaCl, 50 mM Tris-HCl, 5 mM EDTA, 5 mM β-mercaptoethanol, 40% glycerol, pH 7.8.
    EC number3.4.22.17
    Purity≥98% by SDS-PAGE
    Specific activity≥1500 units/mg protein
    Unit definitionOne unit is defined as the amount of enzyme that hydrolyzes 1 pmol. Suc-leu-Leu0Val-Tyr-AMC in 1 min at 25°C using the Calpain Activity Assay Kit, Fluorometric (Cat. No. QIA120). Note: 1 caseinolytic unit = 0.8 fluorogenic units.
    Storage Avoid freeze/thaw
    ≤ -70°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
    Toxicity Harmful
    ReferencesElce, J.S., et al. 1995. Protein Eng. 8, 843.
    Graham-Siegenthaler, K., et al. 1994. J. Biol. Chem. 269, 30457.
    Citation
  • Masako Shimada, et al. (2005) The receptor for parathyroid hormone and parathyroid hormone-related peptide is hydrolyzed and its signaling properties are altered by directly binding the calpain small subunit. Endocrinology 146, 2336-2344.
    		•