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SCT044 BioTracker LYSO-TP Live Cell Dye

Live cell imaging dye for hypochlorous acid (HOCl) targeted to cellular lysosomes used to detect phagocytosis, inflammation and cellular apoptosis.

BioTracker LYSO-TP Live Cell Dye MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Based Assays
SCT044
1 mg  
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Key Applications
Cell Based Assays
Description
Catalogue NumberSCT044
DescriptionBioTracker LYSO-TP Live Cell Dye
OverviewHypochlorous acid (HOCl) is a highly potent reactive oxygen species (ROS) and helps eliminate pathogens in the innate immune system. Mounting evidence indicates that intracellular HOCl plays additional important roles in regulating inflammation and cellular apoptosis. Subcellular detection of HOCL is currently limited due to low concentration, strong oxidization, and short lifespan of the analyte.

The BioTracker LYSO-TP dye is a live cell two-photon green fluorescent “turn-on” imaging probe for HOCL. The probe targets HOCL specifically in the lysosomes of cells. The probe exhibit fast response times, good selectivity, and high sensitivity towards hypochlorous acid in living cells. Acedan was chosen as the fluorescence reporting group due to its excellent photophysical properties resulting from the typical “push−pull” (amineketone) structure. Reaction of the probe with HOCl, which deprotects the oxathiolane/mercaptal group to reveal the ketone, would lead to fluorescence enhancement. To monitor subcellular HOCL levels, morpholine was introduced at a long distance from the reaction site to ensure the probe’s intracellular localization.

Spectral Properties
Absorbance: 375nm
Emission: 500nm

References
Chang YT et al. Development of Targetable Two-Photon Fluorescent Probes to Image Hypochlorous Acid in Mitochondria and Lysosome in Live Cell and Inflamed Mouse. Model. J Am Chem Soc. 2015 May 13;137(18):5930-8.
Alternate Names
  • Live cell imaging probe
Product Information
Detection methodFluorescence
PresentationLyophilized
Applications
ApplicationLive cell imaging dye for hypochlorous acid (HOCl) targeted to cellular lysosomes used to detect phagocytosis, inflammation and cellular apoptosis.
Key Applications
  • Cell Based Assays
Application NotesLive cell fluorescent imaging
Product Usage Statements
Quality AssurancePurity: ≥ 98% confirmed by HNMR, LC-MS and HPLC and elemental analysis
Molar Mass: 357.49 g/mol
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore BioTracker LYSO-TP Live Cell Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Packaging Information
Material Size1 mg
Global Trade Item Number
Catalogue Number GTIN
SCT044 04054839532382