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OP07L Anti-v-Src (Ab-1) Mouse mAb (327)

Anti-v-Src (Ab-1) Mouse mAb (327) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
OP07L
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~60 kDa v-Src and c-Src proteins in NIH/3T3 and HT1080 cells. May appear as a doublet due to phosphorylation.
Catalogue NumberOP07L
Brand Family Calbiochem®
References
ReferencesCourtneidge, S.A. and Heber, A. 1987. Cell 50, 1031.
Cartwright, C.A., et al. 1985. Mol. Cell. Biol. 5,, 2647.
Courtneidge, S.A. 1985. EMBO J. 4, 1471.
Whitman, M., et al. 1985. Nature 315, 239
Bolen, J.B., et al. 1984. Cell 38, 767.
Hunter, T. and Sefton, B. 1980. Proc. Natl. Acad. Sci. 77, 1311.
Collett, M.S. et al. 1979. Proc. Natl. Acad. Sci. 76, 3159.
Collett, M.S. and Erikson, R.L. 1978. Proc. Natl. Acad. Sci. 75, 2021.
Collett, M.S., et al. 1978. Cell 15, 1363.
Product Information
FormLyophilized
FormulationLyophilized from a volatile buffer, 100 µg BSA.
Positive controlNIH/3T3 cells
PreservativeNone
Quality LevelMQ100
Applications
Application ReferencesImmune Kinase Reaction Bolen, J.B., et al. 1985. Proc. Natl. Acad. Sci. 82, 7275. Immunoblotting Lipsich, L.A., et al. 1983. J. Virol. 48, 352. Grandori, C. and Hanfusa, H. 1988. J. Cell Biol. 107, 2125. Immunoprecipitation Grandori, C. and Hanfusa, H. 1988. J. Cell Biol. 107, 2125. Lipsich, L.A., et al. 1983. J. Virol. 48, 352. Original Clone Lipsich, L.A., et al. 1983. J. Virol. 48, 352.
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Not Frozen Sections
Not Immunofluorescence
Not Paraffin Sections
Application NotesFrozen Sections (not recommended)
Immunoblotting (2.5 µg/ml, see application references)
Immunofluorescence (not recommended)
Immunoprecipitation (1 µg/sample or use Cat. No. OP07A; see application references)
Paraffin Sections (not recommended)
Application Commentspp60src may appear as a doublet due to phosphorylation. This antibody can be used to precipitate active Src kinase(see application references). The agarose conjugate, Cat. No. OP07A, is available separately for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenpurified v-Src
Epitopewithin the SH3 domain of v-Src and c-Src
Clone327
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline, pH 7.4, to yield a final concentration of 100 µg/ml. Following reconstitution, refrigerate (4°C) with 0.1% azide for short-term storage or aliquot and freeze (-20°C) for long-term storage. Avoid repeated freeze/thaw cycles of solutions. Lyophilized antibodies should be reconstituted at 4°C with occasional gentle mixing for at least 2 h.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
OP07L 0

Documentation

Anti-v-Src (Ab-1) Mouse mAb (327) MSDS

Title

Safety Data Sheet (SDS) 

Anti-v-Src (Ab-1) Mouse mAb (327) Certificates of Analysis

TitleLot Number
OP07L

References

Reference overview
Courtneidge, S.A. and Heber, A. 1987. Cell 50, 1031.
Cartwright, C.A., et al. 1985. Mol. Cell. Biol. 5,, 2647.
Courtneidge, S.A. 1985. EMBO J. 4, 1471.
Whitman, M., et al. 1985. Nature 315, 239
Bolen, J.B., et al. 1984. Cell 38, 767.
Hunter, T. and Sefton, B. 1980. Proc. Natl. Acad. Sci. 77, 1311.
Collett, M.S. et al. 1979. Proc. Natl. Acad. Sci. 76, 3159.
Collett, M.S. and Erikson, R.L. 1978. Proc. Natl. Acad. Sci. 75, 2021.
Collett, M.S., et al. 1978. Cell 15, 1363.

Citations

Title
  • Meenalakshmi M. Mariappan, et al. (2005) Phospholipase Cγ-Erk Axis in Vascular Endothelial Growth Factor-Induced Eukaryotic Initiation Factor 4E Phosphorylation and Protein Synthesis in Renal Epithelial Cells. Journal of Biological Chemistry 280, 28402-28411.
  • Justin M. Summy, et al. (2005) AP23846, a novel and highly potent Src family kinase inhibitor, reduces vascular endothelial growth factor and interleukin-8 expression in human solid tumor cell lines and abrogates downstream angiogenic processes. Molecular Cancer Therapeutics 4, 1900-1911.
  • Jose G. Trevino, et al. (2005) Expression and activity of Src regulate interleukin-8 expression in pancreatic adenocarcinoma cells: implications for angiogenesis. Cancer Research 65, 7214-7222.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision01-October-2007 RFH
    ApplicationFrozen Sections (not recommended)
    Immunoblotting (2.5 µg/ml, see application references)
    Immunofluorescence (not recommended)
    Immunoprecipitation (1 µg/sample or use Cat. No. OP07A; see application references)
    Paraffin Sections (not recommended)
    DescriptionPurified mouse monoclonal generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63 Ag8.653 myeloma cells (see application references). Recognizes the ~60 kDa Src protein.
    BackgroundThe major translational product of the avian sarcoma virus has been designated pp60src. Avian sarcoma virus pp60src possesses a characteristic protein kinase activity with specificity for tyrosine residues. The mammalian cellular homologue of pp60src, designated pp60c-src, exhibits a similar protein tyrosine kinase activity which can be activated by interaction with polyoma virus-encoded middle T antigen. Immunoprecipitates of middle T antigen also contain phosphatidylinositol kinase activity coinciding with the presence of a 81 kDa (p81) protein. The p81 is phosphorylated by activated pp60c-src and is thought to be the phosphatidylinositol kinase.
    HostMouse
    Immunogenpurified v-Src
    Epitopewithin the SH3 domain of v-Src and c-Src
    Clone327
    IsotypeIgG₁
    Specieshuman, mouse, rat
    Positive controlNIH/3T3 cells
    FormLyophilized
    FormulationLyophilized from a volatile buffer, 100 µg BSA.
    PreservativeNone
    Commentspp60src may appear as a doublet due to phosphorylation. This antibody can be used to precipitate active Src kinase(see application references). The agarose conjugate, Cat. No. OP07A, is available separately for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
    Storage +2°C to +8°C
    Do Not Freeze Ok to freeze
    Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline, pH 7.4, to yield a final concentration of 100 µg/ml. Following reconstitution, refrigerate (4°C) with 0.1% azide for short-term storage or aliquot and freeze (-20°C) for long-term storage. Avoid repeated freeze/thaw cycles of solutions. Lyophilized antibodies should be reconstituted at 4°C with occasional gentle mixing for at least 2 h.
    Toxicity Standard Handling
    ReferencesCourtneidge, S.A. and Heber, A. 1987. Cell 50, 1031.
    Cartwright, C.A., et al. 1985. Mol. Cell. Biol. 5,, 2647.
    Courtneidge, S.A. 1985. EMBO J. 4, 1471.
    Whitman, M., et al. 1985. Nature 315, 239
    Bolen, J.B., et al. 1984. Cell 38, 767.
    Hunter, T. and Sefton, B. 1980. Proc. Natl. Acad. Sci. 77, 1311.
    Collett, M.S. et al. 1979. Proc. Natl. Acad. Sci. 76, 3159.
    Collett, M.S. and Erikson, R.L. 1978. Proc. Natl. Acad. Sci. 75, 2021.
    Collett, M.S., et al. 1978. Cell 15, 1363.
    Citation
  • Meenalakshmi M. Mariappan, et al. (2005) Phospholipase Cγ-Erk Axis in Vascular Endothelial Growth Factor-Induced Eukaryotic Initiation Factor 4E Phosphorylation and Protein Synthesis in Renal Epithelial Cells. Journal of Biological Chemistry 280, 28402-28411.
  • Justin M. Summy, et al. (2005) AP23846, a novel and highly potent Src family kinase inhibitor, reduces vascular endothelial growth factor and interleukin-8 expression in human solid tumor cell lines and abrogates downstream angiogenic processes. Molecular Cancer Therapeutics 4, 1900-1911.
  • Jose G. Trevino, et al. (2005) Expression and activity of Src regulate interleukin-8 expression in pancreatic adenocarcinoma cells: implications for angiogenesis. Cancer Research 65, 7214-7222.
    		•
    Application referencesImmune Kinase Reaction Bolen, J.B., et al. 1985. Proc. Natl. Acad. Sci. 82, 7275. Immunoblotting Lipsich, L.A., et al. 1983. J. Virol. 48, 352. Grandori, C. and Hanfusa, H. 1988. J. Cell Biol. 107, 2125. Immunoprecipitation Grandori, C. and Hanfusa, H. 1988. J. Cell Biol. 107, 2125. Lipsich, L.A., et al. 1983. J. Virol. 48, 352. Original Clone Lipsich, L.A., et al. 1983. J. Virol. 48, 352.