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07-449-AF488 Anti-trimethyl-Histone H3 (Lys27) Antibody, Alexa Fluor® 488 Conjugate

This Anti-trimethyl-Histone H3 (Lys27) Antibody, Alexa Fluor® 488 Conjugate is validated for use in Immunocytochemistry for the detection of trimethyl-Histone H3.

Anti-trimethyl-Histone H3 (Lys27) Antibody, Alexa Fluor® 488 Conjugate MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: HIST3H3, H3FT UniProt Number: Q16695
07-449-AF488
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, H, RICCRbAlexaFluor®488Polyclonal Antibody
Description
Catalogue Number07-449-AF488
DescriptionAnti-trimethyl-Histone H3 (Lys27) Antibody, Alexa Fluor® 488 Conjugate
Alternate Names
  • Histone H3.1t
  • H3/t
  • H3t
  • H3/g
  • trimethyl-Histone (Lys27), Alexa Fluor® 488 Conjugate
Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
References
Product Information
FormatAlexaFluor®488
PresentationPurified rabbit antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-trimethyl-Histone H3 (Lys27) Antibody, Alexa Fluor® 488 Conjugate is validated for use in Immunocytochemistry for the detection of trimethyl-Histone H3.
Key Applications
  • Immunocytochemistry
Application NotesThe unconjugated antibody (Cat. No. 07-449) is shown to be suitable also for immunohistochemistry, immunocytochemistry, immunoprecipitation, multiplex assay, Western blotting, chromatin immunoprecipitation (ChIP), and ChIP-seq applications.
Biological Information
ImmunogenKLH-conjugated, synthetic 2X-branched peptide containing the sequence …AR(Kme3)SAP… in which Kme3 corresponds to trimethyl-lysine at residue 27 of human histone H3.
EpitopeH3K27me3
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityH3K27me3 specificity of the unconjugated antibody (Cat. No. 07-449) has been confirmed using the Absurance™ Histone H3 Antibody Specificity Array (Cat. No. 16-667) and the Absurance™ Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).
IsotypeIgG
Species Reactivity
  • Mouse
  • Human
  • Rat
Species Reactivity NoteHuman, Mouse, Rat. Broad species cross-reactivity expected.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST3H3
  • H3FT
Modifications
  • Methylation
Purification MethodProtein A Purfied
UniProt Number
Molecular Weight~16 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected trimethyl-Histone H3 (Lys27) in A431 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
07-449-AF488 04055977298246

Documentation

Anti-trimethyl-Histone H3 (Lys27) Antibody, Alexa Fluor® 488 Conjugate MSDS

Title

Safety Data Sheet (SDS) 

Anti-trimethyl-Histone H3 (Lys27) Antibody, Alexa Fluor® 488 Conjugate Certificates of Analysis

TitleLot Number
Anti-trimethyl-Histone H3 (Lys27), -Q2623736 Q2623736
Anti-trimethyl-Histone H3 (Lys27), Alexa Fluor® 488 Conjugate 2911833
Anti-trimethyl-Histone H3 (Lys27), Alexa Fluor® 488 Conjugate - 3487544 3487544
Anti-trimethyl-Histone H3 (Lys27), Alexa Fluor® 488 Conjugate - 3677706 3677706
Anti-trimethyl-Histone H3 (Lys27), Alexa Fluor® 488 Conjugate - 3864997 3864997
Anti-trimethyl-Histone H3 (Lys27), Alexa Fluor® 488 Conjugate - 4045886 4045886
Anti-trimethyl-Histone H3 (Lys27), Alexa Fluor® 488 Conjugate - 4095797 4095797