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ABS2206-100UG Anti-phospho-Ubiquitin (Ser57)

Anti-phospho-Ubiquitin (Ser57), Cat. No. ABS2206, is a rabbit polyclonal antibody that detects phospho-Ubiquitin (Ser57) and is tested for use in Affinity Binding Assay and Western Blotting.

ABS2206-100UG
100 μg  
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Overview

Replacement Information
Description
Catalogue NumberABS2206-100UG
DescriptionAnti-phospho-Ubiquitin (Ser57)
Alternate Names
  • Ub
Background InformationUbiquitin (Ub) is initially produced as a 229 amino acids Polyubiquitin-B (UniProt: P0CG47) precursor protein encoded by the UBB gene (Gene ID: 7314) or a 685 amino acids Polyubiquitin-C precursor protein (UniProt: P0CG48) encoded by the UBC gene (Gene ID: 7316) in human. Ub exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Ub is linked covalently via its carboxyl terminus (Gly76) to usually Lys residues in target proteins. In a given target Lys residue can be linked to one single Ub molecule (monoubiquitylated) or to a chain of Ub molecules (polyubiquitylated). In a polyUb chain, Ub molecules can be linked through one of the seven Lys residues (K6, K11, K27, K29, K33, K48, and K63) or through the Ub N-terminus Met1 residue (which generates linear chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked. For example, lysine 6-linked may be involved in DNA repair; lysine 11-linked is involved in endoplasmic reticulum-associated degradation (ERAD) and in cell-cycle regulation, and lysine 29-linked is involved in lysosomal degradation. Lysine 48-linked chains mark proteins for proteasomal degradation, while lysine 63-linked chains are involved in endocytosis, DNA-damage responses, and in signaling leading to activation of the transcription factor NF-kappa-B. Ubiquitin undergoes phosphorylation at the serine 57 by a ubiquitin kinase and this phosphorylation is an important modifier of ubiquitin function, particularly in response to proteotoxic stress. This phosphorylation may also be a deciding factor whether ubiquitin is recycled or degraded during multi-vesicular body sorting on endosomes. (Ref.: Hepowit, NL., et al (2020). eLife 9; e58155; Lee, S., et al. (2017) eLife. 6; e29176).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-phospho-Ubiquitin (Ser57), Cat. No. ABS2206, is a rabbit polyclonal antibody that detects phospho-Ubiquitin (Ser57) and is tested for use in Affinity Binding Assay and Western Blotting.
Key Applications
  • Western Blotting
  • Affinity Binding Assay
Application NotesTested Applications

Affinity Binding Assay: A representative lot of this antibody bound phospho-Ubiquitin (ser57) peptide with a KD of 1.0 x 10-12 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 10 amino acids surrounding phosphoserine 57 of human Ubiquitin.
EpitopeN-terminal half
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRabbit
SpecificityThis rabbit polyclonal antibody specifically detects Ubiquitin phosphorylated on serine 57.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Chimpanzee, Hamster, Mouse, Rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • UBB
Modifications
  • Phosphorylation
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~28 kDa observed for GST-tagged recombinant fragment observed; Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting with a construct containing GST-tagged recombinant fragment of Ubiquitin phosphorylated on serine 57.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected a construct containing GST-tagged recombinant Ubiquitin phosphorylated on serine 57, but did not react with non-phosphorylated construct. (Phospho-Ubiquitin construct: Courtesy of Dr. Jesse Rinehart, Yale University, School of Medicine).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABS2206-100UG 04065267327716