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07-852 Anti-phospho-MEK1 (Thr292) Antibody

Anti-phospho-MEK1 (Thr292) Antibody is an antibody against phospho-MEK1 (Thr292) for use in WB.

Anti-phospho-MEK1 (Thr292) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: MAP Kinases Gene Symbol: MAP2K1, MAPKK1, PRKMK1, MEK1, MKK1 UniProt Number: Q02750
07-852
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-852
Replaces07-348
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-phospho-MEK1 (Thr292) Antibody
Alternate Names
  • MAP Kinase Kinase 1
  • MAPK/ERK Kinase 1
  • MAPKK1
  • MAP2K1
References
Product Information
FormatAffinity Purified
Presentation100 μL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.
Quality LevelMQ100
Applications
ApplicationAnti-phospho-MEK1 (Thr292) Antibody is an antibody against phospho-MEK1 (Thr292) for use in WB.
Key Applications
  • Western Blotting
Application NotesImmunoblot Analysis: A 1:1000 dilution of this lot detected phosphorylated MEK1 (Thr292) in RIPA lysates of untreated A431 cells.
Biological Information
ImmunogenPeptide corresponding to amino acid region encompassing the human, mouse, rat and hamster phospho-MEK1 (Thr292).
HostRabbit
SpecificityRecognizes phospho-MEK1 (Thr292).
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
Gene Symbol
  • MAP2K1
  • MAPKK1
  • PRKMK1
  • MEK1
  • MKK1
Modifications
  • Phosphorylation
Purification MethodImmunoAffinity Purified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q02750 # Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
SIZE: 393 amino acids; 43439 Da
SUBUNIT: Interacts with MORG1 (By similarity). Interacts with Yersinia yopJ.
PTM: Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity. & Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
DISEASE: SwissProt: Q02750 # Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio- cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
SIMILARITY: SwissProt: Q02750 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. & Contains 1 protein kinase domain.
Molecular Weight~44 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by immunoblot.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
07-852 04053252619526

Documentation

Anti-phospho-MEK1 (Thr292) Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-MEK1 (Thr292) Antibody Certificates of Analysis

TitleLot Number
Anti-phospho-MEK1 (Thr292) 3076535
Anti-phospho-MEK1 (Thr292) (Affinity purified Rabbit polyclonal IgG) - 2387484 2387484
Anti-phospho-MEK1 (Thr292) (Affinity purified Rabbit polyclonal IgG) - 2390384 2390384
Anti-phospho-MEK1 (Thr292) (Affinity purified Rabbit polyclonal IgG) 3094655
Anti-phospho-MEK1 (Thr292) (Affinity purified Rabbit polyclonal IgG) 2883129
Anti-phospho-MEK1 (Thr292) (Affinity purified Rabbit polyclonal IgG) - 1967324 1967324
Anti-phospho-MEK1 (Thr292) (Affinity purified Rabbit polyclonal IgG) - 2199557 2199557
Anti-phospho-MEK1 (Thr292) (Affinity purified Rabbit polyclonal IgG) - 2219321 2219321
Anti-phospho-MEK1 (Thr292) (Affinity purified Rabbit polyclonal IgG) - 3692644 3692644
Anti-phospho-MEK1 (Thr292) (Affinity purified Rabbit polyclonal IgG) - DAM1487534 DAM1487534
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