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07-1238 Anti-phospho-MARCKS (Ser152/156) Antibody

This Anti-phospho-MARCKS (Ser152/156) Antibody is validated for use in WB, IH(P) for the detection of phospho-MARCKS (Ser152/156).

Anti-phospho-MARCKS (Ser152/156) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Cytoskeletal Signaling Gene Symbol: MARCKS, MACS, 80K-L, PRKCSL, FLJ14368, phosphomyristin, MRACKS, PKCSL, FLJ90045 UniProt Number: P29966
07-1238
100 µL  
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Overview

Replacement Information
Replacement Information07-1238 is a recommended replacement for AB5879

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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
R, H, MWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-1238
ReplacesAB5879
DescriptionAnti-phospho-MARCKS (Ser152/156) Antibody
Alternate Names
  • Myristoylated alanine-rich C-kinase substrate
  • Protein kinase C substrate, 80 kDa protein, light chain
Background InformationMyristoylated Alanine-Rich C-Kinase Substrate (MARCKS) is a widely expressed substrate of PKC. MARCKS is essential in neuronal development, as mice deficient in it die shortly after birth. Through its basic effector region, MARCKS associates with acidic membrane phospholipids, actin filaments, and calmodulin. Poly (ADP-ribose) also binds the basic effector region, blocking calmodulin binding, membrane association, and phosphorylation by PKC, suggesting that MARCKS may be an effector of the DNA-damage signal transduced by poly (ADP-ribose).
References
Product Information
FormatAffinity Purified
Control
  • A431 Cell Lysate
PresentationPurified rabbit polyclonal IgG in 10 mM Hepes, pH 7.5, with 150 mM NaCl, 100 µg/mL BSA and 50% glycerol.
Quality LevelMQ100
Applications
ApplicationThis Anti-phospho-MARCKS (Ser152/156) Antibody is validated for use in WB, IH(P) for the detection of phospho-MARCKS (Ser152/156).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry(paraffin):
Representative images from a previous lot.
Optimal Staining of MARCKS With Citrate pH 6.0 Epitope Retrieval: Rat Placenta

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenSynthetic peptide corresponding to amino acids surrounding the phosphoSer152/156 of rat MARCKS.
EpitopephosphoSer 152/156
HostRabbit
SpecificityMARCKS, phosphoSer152/156.
IsotypeIgG
Species Reactivity
  • Rat
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis.
Gene Symbol
  • MARCKS
  • MACS
  • 80K-L
  • PRKCSL
  • FLJ14368
  • phosphomyristin
  • MRACKS
  • PKCSL
  • FLJ90045
Modifications
  • Phosphorylation
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P29966 # MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
SIZE: 332 amino acids; 31555 Da
PTM: Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.
SIMILARITY: SwissProt: P29966 ## Belongs to the MARCKS family.
Molecular Weight~80 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western Blot on EGF treated A431 lysates.

Western Blot Analysis: 1:500 dilution of this lot detected phosphorylated MARCKS on 10 μg of EGF treated A431 lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable at -20°C in undiluted for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
07-1238 04053252583667

Documentation

Anti-phospho-MARCKS (Ser152/156) Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-MARCKS (Ser152/156) Antibody Certificates of Analysis

TitleLot Number
Anti-phospho-MARCKS (Ser152/156) - 2121773 2121773
Anti-phospho-MARCKS (Ser152/156) - 1969075 1969075
Anti-phospho-MARCKS (Ser152/156) - 2031362 2031362
Anti-phospho-MARCKS (Ser152/156) - 2280467 2280467
Anti-phospho-MARCKS (Ser152/156) - 2363545 2363545
Anti-phospho-MARCKS (Ser152/156) - 3209741 3209741
Anti-phospho-MARCKS (Ser152/156) - 3308236 3308236
Anti-phospho-MARCKS (Ser152/156) - 3984596 3984596
Anti-phospho-MARCKS (Ser152/156) - NG1605296 NG1605296
Anti-phospho-MARCKS (Ser152/156) - NG1638567 NG1638567
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Life Science Research > Antibodies and Assays > Primary Antibodies