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ABS217 Anti-phospho-LAT Antibody

Detect LAT using this rabbit polyclonal antibody, Anti-phospho-LAT Antibody validated for use in western blotting.

Anti-phospho-LAT Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Immunological Signaling Gene Symbol: LAT UniProt Number: O43561
ABS217
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABS217
Replaces04-467
DescriptionAnti-phospho-LAT Antibody
Alternate Names
  • Linker for activation of T-cells family member 1
  • 36 kDa phospho-tyrosine adapter protein
  • pp36
  • p36-38
Background InformationLAT (Linker for activation of T cells) is a transmembrane adaptor protein that plays an essential role in T cell receptor (TCR) signal transduction pathways. LAT is phosphorylated on multiple tyrosine residues upon TCR engagement, recruits several important signaling proteins to the membrane, and functions as a scaffold for the assembly of numerous signaling complexes.
References
Product Information
FormatAffinity Purified
Control
  • LAT immunoprecipitated from untreated or CD3 treated Jurkat cell lysates.
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect LAT using this rabbit polyclonal antibody, Anti-phospho-LAT Antibody validated for use in western blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: 1 µg/mL from a representative lot detected phospho LAT (Tyr200) in CD3 treated Jurkat cell lysates. However, a decrease in signal was observed against phospho LAT (Tyr200) in untreated Jurkat cell lysates.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the cytoplasmic domain that interacts with GRB2, GRAP2 and PIK3R1 of human LAT phosphorylated at Tyrosine 200.
EpitopeCytoplasmic domain that interacts with GRB2, GRAP2 and PIK3R1
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes the cytoplasmic domain that interacts with GRB2, GRAP2 and PIK3R1 of LAT phosphorylated at Tyrosine 200.
Species Reactivity
  • Human
Species Reactivity NoteDemonstrated to react with Human. Other homologies: Rat, Mouse, and Bovine (89% sequence homology).
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • LAT
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~36 kDa observed. Uncharacterized bands may be observed in some cell lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in LAT immunoprecipitated from untreated or CD3 treated Jurkat cell lysates.

Western Blotting Analysis: 1 µg/mL of this antibody detected phospho LAT (Tyr200) immunoprecipitated from CD treated Jurkat cell lysates. A decrease in signal was observed against phospho LAT (Tyr200) immunoprecipitated from untreated Jurkat cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABS217 04053252906336