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ABE1066-25UL Anti-phospho Exo1 (Ser746)

Anti-phospho Exo1 (Ser746), Cat. No. ABE1066, is a highly specific rabbit polyclonal antibody that detects Exonuclease 1 phosphorylated on Serine 746 and has been tested for use in Dot Blot and Western Blotting.

ABE1066-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberABE1066-25UL
DescriptionAnti-phospho Exo1 (Ser746)
Alternate Names
  • hExo1
  • Exonuclease 1
  • hExoI
Background InformationExonuclease 1 (UniProt: Q9UQ84; also known as hExo1, Exonuclease I, hExoI) is encoded by the EXO1 (also known as EXOI, HEX1) gene (Gene ID: 9156) in human. Exo1 1 is a nuclear enzyme that is shown to colocalize with PCNA to discrete nuclear foci in S-phase. It displays 5' to 3' exonuclease activity as well as an RNase H activity. It is highly expressed in bone marrow, testis and thymus and in fetal liver. It participates in DNA mismatch repair (MMR), micro-mediated end-joining, homologous recombination (HR), and replication. It is shown to preferentially cleave one nucleotide inwards in a double stranded region of forked and nicked DNA flap substrates. Exonuclease 1 contains two subdomains, the NH2-terminal (N) domain and the internal (I) domain that are separated by a spacer region. The N-domain is reported to facilitate binding of Exo1 to the DNA and the I-domain exhibits multiple cysteine and glutamate residues that are required for the binding of magnesium ions. Exonuclease 1 is phosphorylated by ATM or ATR in response to DNA damage and agents that stall DNA replication. Phosphorylation at serine 454, threonine 454 and serine 714 is induced upon DNA-damage following treatment with hydroxyurea. These phosphorylations facilitate destabilization/degradation of the protein. Exo1 also displays a direct and robust interaction with 14-3-3 protein which is achieved via phosphorable serine 746. Phosphorylation of serine 746 is shown to occur under basal cellular conditions. (Ref.: Keijzers, G., et al. (2015). Biosci. Rep. 35(3); e00206; Anderson, SD et a. (2012). DNA Repair (Amst). 11(3): 267-277.
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-phospho Exo1 (Ser746), Cat. No. ABE1066, is a highly specific rabbit polyclonal antibody that detects Exonuclease 1 phosphorylated on Serine 746 and has been tested for use in Dot Blot and Western Blotting.
Key Applications
  • Dot Blot
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected phospho Exo1 (Ser746) in 293T cells transfected with Flag-Exo1(WT) (Courtesy of Dr. Zhongsheng You @ Washington University in St. Louis).

Western Blotting Analysis: A 1:500 dilution from a representative lot detected phospho Exo1 (Ser746) in 293T cells transfected with Flag-Exo1(WT).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 10 amino acids surrounding phosphoserine 746 from the C-terminal region of human Exonuclease 1.
EpitopeC-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Exonuclease 1 phosphorylated on Serine 746. It targets an epitope within 10 amino acids surrounding the phosphoserine 746 residue from the C-terminal region.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Monkey based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • EXOi
  • EXOI
  • HEX1
Modifications
  • Phosphorylation
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~110 kDa observed; 94.10 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Dot Blot with peptide phosphorylated on serine 746.

Dot Blot Analysis: 2.5 to 20 ng of this antibody detected phospho Exo1 (Ser746) in a peptide containing phosphoserine 746.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE1066-25UL 04061841030386