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ABC478 Anti-phospho-CYLD Antibody (Ser418)

ABC478
100 µL  
Purchase on Sigma-Aldrich

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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbSerumPolyclonal Antibody
Description
Catalogue NumberABC478
DescriptionAnti-phospho-CYLD Antibody (Ser418)
Alternate Names
  • Ubiquitin carboxyl-terminal hydrolase CYLD
  • Deubiquitinating enzyme CYLD
  • Ubiquitin thioesterase CYLD
  • Ubiquitin-specific-processing protease CYLD
Background InformationCYLD is a deubiquitinating enzyme that inactivates transcription factor NF-kappaB using TNFRs and is essential for normal cellular homeostasis of skin appendages. Deactivation or mutation of CYLD causes a loss of the deubiquitination activity and increases tumorigenesis. Loss of CYLD specifically results in familial cylindromatosis which is characterized by a predisposition to cylindromas, tumors of skin appendages. CYLD has been shown to cleave the lysine 63-linked polyubiquitin chain from proteins and the inactivity of CYLD has also been noticed in many other human cancers.
References
Product Information
FormatSerum
PresentationRabbit polyclonal serum with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-phospho-CYLD Antibody (Ser418) is validated for use in Western Blotting for the detection of phospho-CYLD (Ser418).
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected phospho-CYLD (Ser418) in 10 µg of human spleen and Jurkat lysate.
Western Blotting Analysis: A representative lot detected phospho-CYLD (Ser418) in PMA and Ionomycin-treated Jurkat cell lysate (Reiley, W., et al. (2005). Mole. Cell Biol. 25(10):3886-3895).
Biological Information
ImmunogenLinear peptide corresponding to human phospho-CYLD (Ser418).
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis antibody detects phospho-CYLD at Ser418. The phosphorylation site of CYLD shares some sequence homology with serine phosphorylation site of c-Cbl.
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • CYLD
  • CYLD1
  • KIAA0849
  • HSPC057
Purification MethodUnpurified
UniProt Number
Molecular Weight~110 kDa observed.
Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho-CYLD (Ser418) in 10 µg of Raji cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABC478 04053252995835

Documentation

Anti-phospho-CYLD Antibody (Ser418) MSDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-CYLD Antibody (Ser418) Certificates of Analysis

TitleLot Number
Anti-phospho-CYLD (Ser418) - Q2454915 Q2454915
Anti-phospho-CYLD (Ser418) - Q2456141 Q2456141

References

Reference overviewPub Med ID
Regulation of the deubiquitinating enzyme CYLD by IkappaB kinase gamma-dependent phosphorylation.
Reiley, William, et al.
Mol. Cell. Biol., 25: 3886-95 (2005)  2004

Show Abstract
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