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ABC80 Anti-phospho AMBRA1 Antibody (Ser52)

Detect Activating molecule in BECN1-regulated autophagy protein 1 using this rabbit polyclonal antibody, Anti-phospho AMBRA1 Antibody (Ser52) validated for use in western blotting & Peptide Inhibition Assay.

Anti-phospho AMBRA1 Antibody (Ser52) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: AMBRA1 UniProt Number: Q9C0C7
ABC80
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, PIARbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABC80
DescriptionAnti-phospho AMBRA1 Antibody (Ser52)
Alternate Names
  • Activating molecule in BECN1-regulated autophagy protein 1
  • Ambra
  • Ambra-1
Background InformationActivating molecule in BECN1-regulated autophagy protein 1 (AMBRA1) interacts with BECN1, likely forming a complex with BECN1 and PIK3C3. AMBRA1 is involved in autophagy by controlling protein turnover during neuronal development, and in regulating differentiation, survival and proliferation of normal cells.
References
Product Information
FormatAffinity Purified
Control
  • HEK293 cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Activating molecule in BECN1-regulated autophagy protein 1 using this rabbit polyclonal antibody, Anti-phospho AMBRA1 Antibody (Ser52) validated for use in western blotting & Peptide Inhibition Assay.
Key Applications
  • Western Blotting
  • Peptide Inhibition Assay
Application NotesWestern Blotting/Peptide Inhibition Analysis: 2 µg/mL from a representative lot detected phospho AMBRA1 (Ser52) in WB/PIA.

Western Blotting Analysis: A representative lot from an independent laboratory detected phospho AMBRA1 (Ser52) in HEK293 cell lysates trated without Torin1 or EBS and demonstrated a loss of signal in HEK293 cell lysates treated with Torin1 or EBS (Nazio, F., et al. (2013). Nat Cell Biol.15(4):406-416.).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human AMBRA1 phosphorylated at Ser52.
EpitopePhosphorylated Ser52
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes AMBRA1 phosphorylated at Ser52.
Species Reactivity
  • Human
Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Mouse, Bovine, and Rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • AMBRA1
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~150 kDa observed. Uniprot describes 6 isoforms from 83 kDa to 143 kDa. This protein may be observed at higher molecular weights possibly due to hyperphosphorylation (Nazio, F., et al. (2013). Nat Cell Biol. [Epub ahead of print].).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected phospho AMBRA1 (Ser52) in 10 µg of HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABC80 04053252915987

Documentation

Anti-phospho AMBRA1 Antibody (Ser52) MSDS

Title

Safety Data Sheet (SDS) 

Anti-phospho AMBRA1 Antibody (Ser52) Certificates of Analysis

TitleLot Number
Anti-phospho AMBRA1 (Ser52) - 2564453 2564453
Anti-phospho AMBRA1 (Ser52) - 3440802 3440802
Anti-phospho AMBRA1 (Ser52) - 3590107 3590107
Anti-phospho AMBRA1 (Ser52) - Q2227355 Q2227355
Anti-phospho AMBRA1 (Ser52) -2606921 2606921
Anti-phospho AMBRA1 (Ser52) -2686107 2686107
Anti-phospho AMBRA1 (Ser52) -2774126 2774126
Anti-phospho AMBRA1 (Ser52) Polyclonal Antibody 2905400
Anti-phospho AMBRA1 (Ser52) Polyclonal Antibody 3105787