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PC435 Anti-p19ARF (54-75) (Ab-1) Rabbit pAb

This Anti-p19ARF (54-75) (Ab-1) Rabbit pAb is validated for use in Immunoblotting for the detection of p19ARF (54-75) (Ab-1).

Anti-p19ARF (54-75) (Ab-1) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
PC435
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
MRbPolyclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~19 kDa p19ARF protein in CTLL2 cells and mouse embryo fibroblasts.
Catalogue NumberPC435
Brand Family Calbiochem®
Application Data
Detection of mouse p19ARF by immunoblotting. Sample: Whole cell lysate from wild-type mouse embryonic fibroblasts (lanes 1 and 2) and whole cell lysate from mouse embtyonic fibroblasts bearing a p19ARF gene knockout as a negative control (lane 3). Primary antibody: Anti-p19ARF (54-75) (Ab-1) Rabbit pAb (Cat. No. PC435) (1 µg/ml). Detection: chemiluminescence.
References
ReferencesDe Stanchina, E., et al. 1998. Genes Dev. 12, 2434.
Larson, C.J., 1998. Bull. Cancer. 85, 304.
Radfar, A., et al. 1998. Proc. Natl. Acad Sci USA. 95, 13194.
Zindy, F., et al. 1998. Genes Dev. 12, 2424.
Product Information
FormLiquid
FormulationIn PBS, 1% BSA.
Positive controlMouse embryo fibroblasts or CTLL2 cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (0.5-1 µg/ml)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids 54-75 of mouse p19ARF, conjugated to KLH
ImmunogenMouse
HostRabbit
IsotypeIgG
Species Reactivity
  • Mouse
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC435 0

Documentation

Anti-p19ARF (54-75) (Ab-1) Rabbit pAb MSDS

Title

Safety Data Sheet (SDS) 

Anti-p19ARF (54-75) (Ab-1) Rabbit pAb Certificates of Analysis

TitleLot Number
PC435

References

Reference overview
De Stanchina, E., et al. 1998. Genes Dev. 12, 2434.
Larson, C.J., 1998. Bull. Cancer. 85, 304.
Radfar, A., et al. 1998. Proc. Natl. Acad Sci USA. 95, 13194.
Zindy, F., et al. 1998. Genes Dev. 12, 2424.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision10-October-2007 RFH
ApplicationImmunoblotting (0.5-1 µg/ml)
Application Data
Detection of mouse p19ARF by immunoblotting. Sample: Whole cell lysate from wild-type mouse embryonic fibroblasts (lanes 1 and 2) and whole cell lysate from mouse embtyonic fibroblasts bearing a p19ARF gene knockout as a negative control (lane 3). Primary antibody: Anti-p19ARF (54-75) (Ab-1) Rabbit pAb (Cat. No. PC435) (1 µg/ml). Detection: chemiluminescence.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~19 kDa p19ARF protein.
BackgroundIn humans, the multiple tumor suppressor 1 locus (MTS1) encodes two unrelated genes, p16INK4a and p19ARF. Although both act as cell proliferation inhibitors, their mechanisms of action are different. p19ARF works as part of a p53-dependant pathway to counter uncontrolled proliferation and oncogenic signals. Mice lacking the p19ARF gene rapidly develop a broad spectrum of tumors. This result indicates that p19ARF is an important tumor suppressor. In addition, p19ARF helps prevent transforming signals from various oncoproteins via the p53 regulatory loop. These studies and others display the importance of p19ARF in cell cycle control and tumor suppression. p19ARF recognizes in vitro translated murine p19ARF and a ~19 kDa band in murine CTLL2 cells.
HostRabbit
Immunogen speciesMouse
Immunogena synthetic peptide corresponding to amino acids 54-75 of mouse p19ARF, conjugated to KLH
IsotypeIgG
Speciesmouse
Positive controlMouse embryo fibroblasts or CTLL2 cells
FormLiquid
FormulationIn PBS, 1% BSA.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesDe Stanchina, E., et al. 1998. Genes Dev. 12, 2434.
Larson, C.J., 1998. Bull. Cancer. 85, 304.
Radfar, A., et al. 1998. Proc. Natl. Acad Sci USA. 95, 13194.
Zindy, F., et al. 1998. Genes Dev. 12, 2424.