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ABE1042 Anti-monomethyl Histone H3 Antibody (Lys18)

Detect methylated Histone H3 using this rabbit polyclonal antibody, Anti-monomethyl Histone H3 Antibody (Lys18) validated for use in western blotting & Dot Blot.

Anti-monomethyl Histone H3 Antibody (Lys18) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones UniProt Number: AAA34659
ABE1042
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, YeastChIP, WB, DBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE1042
DescriptionAnti-monomethyl Histone H3 Antibody (Lys18)
Alternate Names
  • Yeast (S.cerevisiae) partial histone 3 (H3) gene
  • H3K18me1
Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect methylated Histone H3 using this rabbit polyclonal antibody, Anti-monomethyl Histone H3 Antibody (Lys18) validated for use in western blotting & Dot Blot.
Key Applications
  • Chromatin Immunoprecipitation (ChIP)
  • Western Blotting
  • Dot Blot
Application NotesWestern Blotting Analysis: A representative lot detected monomethyl Histone H3 (Lys18) in yeast whole cell extracts, which express wild-type monomethyl Histone H3 (Lys18) and not mutant monomethyl Histone H3 (Lys18Ala) (Prof. F. Howe from the laboratory of Prof. J. Mellor, University of Oxford.).

Dot Blot Analysis: A representative lot detected monomethyl Histone H3 (Lys18) peptide and not unmodified Histone H3 (Prof. F. Howe from the laboratory of Prof. J. Mellor, University of Oxford.).

Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated monomethyl Histone H3 (Lys18) in 1.6X 10E7 yeast cell equivalents (Prof. F. Howe from the laboratory of Prof. J. Mellor, University of Oxford.).
Biological Information
ImmunogenLinear peptide corresponding to yeast Histone H3 monomethylated at Lys18.
HostRabbit
Species Reactivity
  • Human
  • Yeast
Antibody TypePolyclonal Antibody
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~17 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa acid extract.

Western Blotting Analysis: 1:1,000 dilution of this antibody detected monomethyl Histone H3 (Lys18) in 10 µg of HeLa acid extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE1042 04053252928741