Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ABS310 Anti-hCE-1 Antibody

ABS310
100 µL  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbSerumPolyclonal Antibody
Description
Catalogue NumberABS310
DescriptionAnti-hCE-1 Antibody
Alternate Names
  • Carboxylesterase 1
  • Liver carboxylesterase 1
  • Acyl-coenzyme A:cholesterol acyltransferase
  • ACAT
  • Brain carboxylesterase hBr1
  • CE-1
  • hCE-1
  • Cocaine carboxylesterase
  • Egasyn
  • HMSE
  • Methylumbelliferyl-acetate deacetylase 1
  • Monocyte/macrophage serine esterase
  • Retinyl ester hydrolase
  • REH
  • Serine esterase 1
  • Triacylglycerol hydrolase
  • TGH
Background InformationCarboxylesterase 1 (hCE-1) is also known as Liver carboxylesterase 1, Acyl-coenzyme A:cholesterol acyltransferase, (ACAT), Cocaine carboxylesterase, Methylumbelliferyl-acetate deacetylase 1, Monocyte/macrophage serine esterase, Retinyl ester hydrolase, (REH), Serine esterase 1, and Triacylglycerol hydrolase (TGH). hCE-1 is involved in: the detoxification of xenobiotics and the activation of ester and amide prodrugs. hCE-1 functions to hydrolize the methyl ester group of cocaine to form benzoylecgonine, catalyze the transesterification of cocaine to form cocaethylene and the ethyl esterification of oleic acid to ethyloleate, and is activated by CHAPS. hCE-1 is expressed mostly in liver with lower levels found in the heart and lung.
References
Product Information
FormatSerum
Control
  • HepG2 cell lysate
PresentationRabbit polyclonal serum with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Carboxylesterase 1 using this rabbit polyclonal antibody, Anti-hCE-1 Antibody validated for use in western blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected hCE-1 in 10 µg of human liver tissue lysate.
Western Blotting Analysis: A representative lot from an independent laboratory detected hCE-1 in hCE-1 transfected U373MG cell lysates, and demonstrated a loss of signal in non-transfected and hCE-2 transfected U373MG cell lysates (Prof. P. Potter, St. Jude Children's Research Hospital, Memphis.).
Biological Information
ImmunogenLinear peptide corresponding to human hCE-1.
HostRabbit
SpecificityThis antibody does not cross react with human CE-2 (Prof. P. Potter, St. Jude Children's Research Hospital, Memphis).
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • CES1
  • CES2
  • SES1
Purification MethodUnpurified
UniProt Number
Molecular Weight~58 kDa observed. A homodimeric trimer of three hCE-1 molecules may be observed at ~190 kDa in some cell lysates (Prof. P. Potter, St. Jude Children's Research Hospital, Memphis.).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected hCE-1 in 10 µg of HepG2 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABS310 04053252909955

Documentation

Anti-hCE-1 Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-hCE-1 Antibody Certificates of Analysis

TitleLot Number
Anti-hCE-1 - Q2197817 Q2197817

References

Reference overviewPub Med ID
Nerve agent hydrolysis activity designed into a human drug metabolism enzyme.
Hemmert, Andrew C, et al.
PLoS ONE, 6: e17441 (2011)  2010

Show Abstract
21445272 21445272
An improved human carboxylesterase for enzyme/prodrug therapy with CPT-11.
Wierdl, M, et al.
Cancer Gene Ther., 15: 183-92 (2008)  2008

Show Abstract
18188187 18188187