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07-369-I Anti-dimethyl Histone H3 Antibody (Lys36)

Anti-dimethyl Histone H3 Antibody (Lys36) is a highly specific rabbit polyclonal antibody, that targets Histone H3 & has been tested in western blotting, Dot Blot & Chromatin IP (ChIP).

Anti-dimethyl Histone H3 Antibody (Lys36) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HIST3H3, H3FT UniProt Number: Q16695
07-369-I
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, DB, ChIPRbSerumPolyclonal Antibody
Description
Catalogue Number07-369-I
DescriptionAnti-dimethyl Histone H3 Antibody (Lys36)
Alternate Names
  • Histone H3.1t
  • H3/t/ H3t/ H3/g
Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
References
Product Information
FormatSerum
PresentationRabbit polyclonal serum with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-dimethyl Histone H3 Antibody (Lys36) is a highly specific rabbit polyclonal antibody, that targets Histone H3 & has been tested in western blotting, Dot Blot & Chromatin IP (ChIP).
Key Applications
  • Western Blotting
  • Dot Blot
  • Chromatin Immunoprecipitation (ChIP)
Application NotesDot Blot Analysis: A 1:10,000 dilution from a representative lot detected dimethyl Histone H3 (Lys36), but not unmethylated Histone H3 or other peptides corresponding to modified histones.

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot imunoprecipitated dimethyl Histone H3 (Lys36) in HeLa cell lysate.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to a region near the N-terminus of human dimethyl Histone H3 methylated at Lys36.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
Species Reactivity
  • Human
Species Reactivity NotePredicted to react with Vertebrates based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST3H3
  • H3FT
Purification MethodUnpurified
UniProt Number
Molecular Weight~17 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa acid extract.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected dimethyl Histone H3 (Lys36) in 10 µg HeLa acid extract and demonstrated a loss of signal in 0.5 µg of unmethylated recombinant Histone H3.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
07-369-I 04053252918643

Documentation

Anti-dimethyl Histone H3 Antibody (Lys36) MSDS

Title

Safety Data Sheet (SDS) 

Anti-dimethyl Histone H3 Antibody (Lys36) Certificates of Analysis

TitleLot Number
Anti-dimethyl Histone H3 (Lys36) 2475921
Anti-dimethyl Histone H3 (Lys36) 3072328
Anti-dimethyl Histone H3 (Lys36) - 3193855 3193855
Anti-dimethyl Histone H3 (Lys36) - 3425572 3425572
Anti-dimethyl Histone H3 (Lys36) - 3856698 3856698
Anti-dimethyl Histone H3 (Lys36) - 4131166 4131166
Anti-dimethyl Histone H3 (Lys36) - Q2226882 Q2226882
Anti-dimethyl Histone H3 (Lys36) -2522242 2522242
Anti-dimethyl Histone H3 (Lys36) -2550070 2550070
Anti-dimethyl Histone H3 (Lys36) -2581791 2581791
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