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OP15 Anti-c-ErbB2/c-Neu (Ab-3) Mouse mAb (3B5)

Anti-c-ErbB2/c-Neu (Ab-3), mouse monoclonal, clone 3B5, recognizes the ~185 kDa c-ErbB2/c-Neu protein in HEK293 cells. It is validated for WB, IF, IP, and IHC on frozen and paraffin sections.

Anti-c-ErbB2/c-Neu (Ab-3) Mouse mAb (3B5) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-HER2, Anti-c-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus

OP15
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, MMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
OP15-100UG Plastová ampulka 100 μg
Description
OverviewRecognizes the ~185 kDa c-ErbB2/c-Neu protein in HEK293 cells.
Catalogue NumberOP15
Brand Family Calbiochem®
SynonymsAnti-HER2, Anti-c-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus
Application Data
Detection of human c-ErbB2/c-Neu by immunoblotting. Sample: Whole cell lysate from HEK293 cells. Primary antibody: Anti-c-ErbB2/c-Neu (Ab-3) Mouse mAb (3B5) (Cat. No. OP15) (1 µg/ml). Detection: chemiluminescence.
References
ReferencesMamcs, J.R., et al. 1994. Ann. Surg. 219, 332.
Tetu, B., et al. 1994. Cancer 73, 2359.
DiFiore, P.P., et al. 1987. Science 237, 178.
Slamon, D.J., et al. 1987. Science 235, 177.
Varley, J.M., et al. 1987. Oncogene 1, 423.
Bargmann, C.I., et al. 1986. Nature 319, 226.
Yamamoto, T., et al. 1986. Nature 319, 230.
Blick, M., et al. 1984. Blood 64, 1234.
Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.
Product Information
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
Negative controlHepG2 cells or normal skin
Positive controlSK-BR-3 cells or breast carcinoma tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesParaffin Sections Meissner, K., et al. 1990. Am. J. Pathol. 137, 1305. Van De Vijver, J.J., et al. 1988. New Engl. J. Med. 319, 1239. Original Clone Van De Vijver, J.J., et al. 1988. New Engl. J. Med. 319, 1239. Immunohistochemistry, Immunoblotting Zhau, H.E., et al. 1990. Mol. Carcinogen. 3, 254. Immunoprecipitation Van De Vijver, J.J., et al. 1988. New Engl. J. Med. 319, 1239.
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Paraffin Sections
Application NotesFrozen Sections (2.5 µg/ml)
Immunoblotting (2-3 µg/ml, see application references)
Immunofluorescence (2.5 µg/ml)
Immunoprecipitation (1 µg/sample, see application references)
Paraffin Sections (2.5 µg/ml, pressure cooker, heat or trypsin pre-treatment required, see application references)
Application CommentsFor paraffin sections, we recommend pre-treating with a pressure cooker, but trypsin and heat will also work well. Including 0.05% saponin during staining of paraffin sections may help reduce background. In one study ductal carcinomas in situ displaying large-cell, comedo growth type, were stained while none of 16 ductal carcinomas in situ of small-cell, papillary, or cribriform growth type were stained by this antibody. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide (TAENPEYLGLDVPV) corresponding to amino acids 1242-1255 from the C-terminal domain of human c-ErbB2/c-Neu
ImmunogenHuman
Clone3B5
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
OP15-100UG 04055977224658

Documentation

Anti-c-ErbB2/c-Neu (Ab-3) Mouse mAb (3B5) MSDS

Title

Safety Data Sheet (SDS) 

Anti-c-ErbB2/c-Neu (Ab-3) Mouse mAb (3B5) Certificates of Analysis

TitleLot Number
OP15

References

Reference overview
Mamcs, J.R., et al. 1994. Ann. Surg. 219, 332.
Tetu, B., et al. 1994. Cancer 73, 2359.
DiFiore, P.P., et al. 1987. Science 237, 178.
Slamon, D.J., et al. 1987. Science 235, 177.
Varley, J.M., et al. 1987. Oncogene 1, 423.
Bargmann, C.I., et al. 1986. Nature 319, 226.
Yamamoto, T., et al. 1986. Nature 319, 230.
Blick, M., et al. 1984. Blood 64, 1234.
Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.

Citations

Title
  • Peter Nagy, et al. (2005) Decreased Accessibility and Lack of Activation of ErbB2 in JIMT-1 ,a Herceptin-Resistant ,MUC4-Expressing Breast Cancer Cell Line. Cancer Research 65, 473-482.
  • Rita Nahta, et al. (2005) Insulin-like growth factor-I receptor/human epidermal growth factor receptor 2 heterodimerization contributes to trastuzumab resistance of breast cancer cells. Cancer Research 65, 11118-11128.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision02-October-2007 RFH
    SynonymsAnti-HER2, Anti-c-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus
    ApplicationFrozen Sections (2.5 µg/ml)
    Immunoblotting (2-3 µg/ml, see application references)
    Immunofluorescence (2.5 µg/ml)
    Immunoprecipitation (1 µg/sample, see application references)
    Paraffin Sections (2.5 µg/ml, pressure cooker, heat or trypsin pre-treatment required, see application references)
    Application Data
    Detection of human c-ErbB2/c-Neu by immunoblotting. Sample: Whole cell lysate from HEK293 cells. Primary antibody: Anti-c-ErbB2/c-Neu (Ab-3) Mouse mAb (3B5) (Cat. No. OP15) (1 µg/ml). Detection: chemiluminescence.
    DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Sp2/0.Ag14 cells (see application references). Recognizes the ~185 kDa c-Erb2/c-Neu protein.
    BackgroundAmplification of the human proto-oncogene c-neu has been reported to occur in approximately 30% of breast carcinomas and measurement of the amplification has been proposed as a useful predictor of disease prognosis. Gene amplification and the resulting overexpression of proto-oncogene encoded p185c-neu is thought to transform cells by chronically stimulating signal transduction pathways and in model systems overexpression of human c-neu induces cellular transformation. In the rat model system, a point mutation has been located within the transmembrane domain of the c-neu oncogene. neu (from neuroglioblastomas) is also referred to as erbB-2 (from erythroblastosis virus) and HER2 (for Human Epidermal Growth Factor Receptor) and was the second of four members of the EGF receptor family to be described.
    HostMouse
    Immunogen speciesHuman
    Immunogena synthetic peptide (TAENPEYLGLDVPV) corresponding to amino acids 1242-1255 from the C-terminal domain of human c-ErbB2/c-Neu
    Clone3B5
    IsotypeIgG₁
    Specieshuman, mouse
    Positive controlSK-BR-3 cells or breast carcinoma tissue
    Negative controlHepG2 cells or normal skin
    FormLiquid
    FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsFor paraffin sections, we recommend pre-treating with a pressure cooker, but trypsin and heat will also work well. Including 0.05% saponin during staining of paraffin sections may help reduce background. In one study ductal carcinomas in situ displaying large-cell, comedo growth type, were stained while none of 16 ductal carcinomas in situ of small-cell, papillary, or cribriform growth type were stained by this antibody. Antibody should be titrated for optimal results in individual systems.
    Storage +2°C to +8°C
    Do Not Freeze Yes
    Toxicity Standard Handling
    ReferencesMamcs, J.R., et al. 1994. Ann. Surg. 219, 332.
    Tetu, B., et al. 1994. Cancer 73, 2359.
    DiFiore, P.P., et al. 1987. Science 237, 178.
    Slamon, D.J., et al. 1987. Science 235, 177.
    Varley, J.M., et al. 1987. Oncogene 1, 423.
    Bargmann, C.I., et al. 1986. Nature 319, 226.
    Yamamoto, T., et al. 1986. Nature 319, 230.
    Blick, M., et al. 1984. Blood 64, 1234.
    Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.
    Citation
  • Peter Nagy, et al. (2005) Decreased Accessibility and Lack of Activation of ErbB2 in JIMT-1 ,a Herceptin-Resistant ,MUC4-Expressing Breast Cancer Cell Line. Cancer Research 65, 473-482.
  • Rita Nahta, et al. (2005) Insulin-like growth factor-I receptor/human epidermal growth factor receptor 2 heterodimerization contributes to trastuzumab resistance of breast cancer cells. Cancer Research 65, 11118-11128.
    		•
    Application referencesParaffin Sections Meissner, K., et al. 1990. Am. J. Pathol. 137, 1305. Van De Vijver, J.J., et al. 1988. New Engl. J. Med. 319, 1239. Original Clone Van De Vijver, J.J., et al. 1988. New Engl. J. Med. 319, 1239. Immunohistochemistry, Immunoblotting Zhau, H.E., et al. 1990. Mol. Carcinogen. 3, 254. Immunoprecipitation Van De Vijver, J.J., et al. 1988. New Engl. J. Med. 319, 1239.