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MABS1300 Anti-asparagine synthetase Antibody, clone Z5808

This mouse monoclonal Anti-asparagine synthetase, clone Z5808, Cat. No. MABS1300 has been tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting for the detection of human Asparagine Synthetase.

Anti-asparagine synthetase Antibody, clone Z5808 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: ASNS, TS11 UniProt Number: P08243
MABS1300
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, WB, ICC, IH(P), ELISAMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1300
DescriptionAnti-asparagine synthetase Antibody, clone Z5808
Alternate Names
  • Asparagine synthetase [glutamine-hydrolyzing]
  • Cell cycle control protein TS11
  • Glutamine-dependent asparagine synthetase
Background InformationAsparagine synthetase [glutamine-hydrolyzing] (UniProt: P08243; also known as EC:6.3.5.4, Cell cycle control protein TS11, Glutamine-dependent asparagine synthetase) is encoded by the ASNS (also known as TS11) gene (Gene ID: 440) in human. Asparagine synthetase catalyzes the biosynthesis of L-asparagine from L-aspartate in an ATP-dependent manner. It contains one asparagine synthetase domain and one glutamine amidotransferase type-2 domain. ASNS transcription is enhanced by amino acid deprivation via a signal transduction pathway that senses amino acid availability. Mutations in ASNS gene are reported to result in asparagine synthetase deficiency that is characterized by microcephaly, severely delayed psychomotor development, progressive encephalopathy, and hyperekplexic activity. There has been much interest in the development of asparagine synthetase inhibitors for treatment of asparaginase-resistance leukemias and levels of asparagine synthetase have been considered as a potential biomarker in ovarian cancers.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in PBS with 0.01% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis mouse monoclonal Anti-asparagine synthetase, clone Z5808, Cat. No. MABS1300 has been tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting for the detection of human Asparagine Synthetase.
Key Applications
  • Flow Cytometry
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry (Paraffin)
  • ELISA
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected asparagine synthetase in human tonsil and human uterus tissues.

Immunocytochemistry Analysis: A representative lot detected asparagine synthetase in Immunocytochemistry applications (Kusano-Arai, O., et. al. (2012). Hybridoma (Larchmt). 31(5):325-32).

Flow Cytometry Analysis: A representative lot detected asparagine synthetase in Flow Cytometry applications (Kusano-Arai, O., et. al. (2012). Hybridoma (Larchmt). 31(5):325-32).

ELISA Analysis: A representative lot detected asparagine synthetase in ELISA applications (Kusano-Arai, O., et. al. (2012). Hybridoma (Larchmt). 31(5):325-32).

Western Blotting Analysis: A representative lot detected asparagine synthetase in Western Blotting applications (Kusano-Arai, O., et. al. (2012). Hybridoma (Larchmt). 31(5):325-32).
Biological Information
ImmunogenN-asparagine synthetase-budded virus.
CloneZ5808
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone Z5808 detects Asparagine synthetase in human creast cancer, human tonsil, and human uterus. It targets an epitope within 50 amino acids from the N-terminal end.
IsotypeIgG2aκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ASNS
  • TS11
Purification MethodAmmonium Sulfate
UniProt Number
Molecular Weight64.37 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in human breast cancer tissues.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected asparagine synthetase in human breast cancer tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS1300 04054839198939