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MABN2552-25UL Sigma-AldrichAnti-alpha B-crystallin Antibody, clone aB1788G7G6

Anti-alpha B-crystallin, clone aB1788G7G6, Cat. No. MABN2552, is a highly specific mouse monoclonal antibody that targets Alpha-crystallin B chain and has been tested for use in ELISA, Immunofluorescence, Immunohistochemistry, Inhibition Assay, and Western Blotting.

Anti-alpha B-crystallin Antibody, clone aB1788G7G6 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References

Research Category: Neuroscience Gene Symbol: CRYAB, CRYA2, HSOB5 UniProt Number: P02511

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Overview

Replacement Information

Key Spec Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M	ELISA, IF, IHC, Inhibition, WB	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MABN2552-25UL
Description	Anti-alpha B-crystallin Antibody, clone aB1788G7G6
Alternate Names	Alpha-crystallin B chain Alpha(B)-crystallin Heat shock protein beta-5 HspB5 Renal carcinoma antigen NY-REN-27 Rosenthal fiber component
Background Information	Alpha-crystallin B chain (UniProt: P02511; also known as Alpha(B)-crystallin, Heat shock protein beta-5, HspB5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component) is encoded by the CRYAB (also known as CRYA2, HSOB5) gene (Gene ID: 1410) in human. Alpha-crystallin is a small heat shock protein that is composed of A and B subunits that display about 55% sequence homology. They both serve as molecular chaperones. They are generally present in a molar ratio of 3:1 (alphaA:alphaB). AlphaB is a stress-inducible form that is present in lens, retina, heart and skeletal muscle, and kidney. However, alpha, which is non-stress inducible form is present mainly in lens. Higher expression of alphaB has also been reported in several cancers, including gliomas, renal cell carcinoma, and metaplastic breast carcinomas. Phosphorylation of alphaB is shown to reduce its oligomerization and its anti-apoptotic activities. As a negative regulator of inflammation, it is shown to protect against multiple sclerosis and block ischemic injury, brain stroke, and spinal cord contusion injury in animal models. Mutations in CRYAB gene is reported to cause myofibrillar myopathies characterized at ultrastructural level by disintegration of the sarcomeric Z disk and myofibrils. (Ref.: Nahomi, RB., et al. (2019). J. Immunol. Methods. 467; 37-47).

References

Product Information
Format	Purified
Presentation	Purified mouse monoclonal antibody IgG1 in PBS without preservatives.

Applications
Application	Anti-alpha B-crystallin, clone aB1788G7G6, Cat. No. MABN2552, is a highly specific mouse monoclonal antibody that targets Alpha-crystallin B chain and has been tested for use in ELISA, Immunofluorescence, Immunohistochemistry, Inhibition Assay, and Western Blotting.
Key Applications	ELISA Immunofluorescence Immunohistochemistry Inhibition Western Blotting
Application Notes	Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected alpha B-crystallin in mouse eye and human retina tissue lysate. Immunohistochemistry Analysis: A representative lot detected alpha B-crystallin in Immunohistochemistry applications (Nahomi, R.B., et. al. (2019). J. Immunol. Methods. 467; 37-47). ELISA Analysis: A representative lot detected alpha B-crystallin in ELISA applications (Nahomi, R.B., et. al. (2019). J. Immunol. Methods. 467; 37-47). Inhibition Analysis: A representative lot inhibited the chaperone and anti-apoptotic activities of alpha B-crystallin. (Nahomi, R.B., et. al. (2019). J. Immunol. Methods. 467; 37-47). Immunofluorescence Analysis: A representative lot detected alpha B-crystallin in Immunofluorescence applications (Nahomi, R.B., et. al. (2019). J. Immunol. Methods. 467; 37-47). Western Blotting Analysis: A representative lot detected alpha B-crystallin in Western Blotting applications (Nahomi, R.B., et. al. (2019). J. Immunol. Methods. 467; 37-47).

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to 21 amino acids from the N-terminal half of human Alpha(B)-crystallin.
Clone	aB1788G7G6
Concentration	Please refer to lot specific datasheet.
Host	Mouse
Specificity	Clone aB1788G7G6 is a mouse monoclonal antibody that detects Alpha(B)-crystallin. It targets an epitope within 21 amino acids within the N-terminal half.
Isotype	IgG1κ
Species Reactivity	Human Mouse
Species Reactivity Note	Human, Mouse. Predicted to react with Bovine, Monkey, Rat based on 100% sequence homology.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_001276736
Gene Symbol	CRYAB CRYA2 HSOB5
Purification Method	Protein G purified
UniProt Number	P02511
Molecular Weight	~20 kDa observed; 20.16 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in mouse retina tissue lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected alpha B-crystallin in mouse retina tissue lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	25 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
MABN2552-25UL	04061841369554

Documentation

Anti-alpha B-crystallin Antibody, clone aB1788G7G6 MSDS

Title
Safety Data Sheet (SDS)

Anti-alpha B-crystallin Antibody, clone aB1788G7G6 Certificates of Analysis

Title	Lot Number
Anti-alpha B-crystallin, clone aB1788G7G6 - Q3380060	Q3380060

References

Reference overview	Pub Med ID
A monoclonal antibody targeted to the functional peptide of αB-crystallin inhibits the chaperone and anti-apoptotic activities. Nahomi, RB; Nandi, SK; Nagaraj, RH J Immunol Methods 467 37-47 2019 Show Abstract αB-Crystallin is a member of the small heat shock protein family. It is a molecular chaperone and an anti-apoptotic protein. Previous studies have shown that the peptide (73DRFSVNLDVKHFSPEELKVKV93, hereafter referred to as peptain-1) from the core domain of αB-crystallin exhibits both chaperone and anti-apoptotic properties similar to the parent protein. We developed a mouse monoclonal antibody against peptain-1 with the aim of blocking the functions of αB-crystallin. The antibody reacted with peptain-1, it did not react with the chaperone peptide of αA-crystallin. The antibody strongly reacted with human recombinant αB-crystallin but weakly with Hsp20; it did not react with αA-crystallin or Hsp27. The antibody specifically reacted with αB-crystallin in human and mouse lens proteins but not with αA-crystallin. The antibody reacted with αB-crystallin in human lens epithelial cells, human retinal endothelial cells, and with peptain-1 in peptain-1-transduced cells. Unlike the commercial antibodies against αB-crystallin, the antibody against peptain-1 inhibited the chaperone and anti-apoptotic activities of peptain-1. The antibody might find use in inhibiting αB-crystallin's chaperone and anti-apoptotic activities in diseases where αB-crystallin is a causative or contributing factor.	30738041

Reference overview

Pub Med ID

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