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CA1026 Anti-Wilm's Tumor Protein Rabbit pAb

This Anti-Wilm's Tumor Protein Rabbit pAb is validated for use in Immunoblotting, Paraffin Sections, Immunoprecipitation for the detection of Wilm's Tumor Protein.

Anti-Wilm's Tumor Protein Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-WT-1

CA1026
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RRbPolyclonal Antibody
Description
OverviewRecognizes the ~47-55 kDa Wilm's tumor protein in Wilm's tumor tissue.
Catalogue NumberCA1026
Brand Family Calbiochem®
SynonymsAnti-WT-1
Application Data
Detection of Wilm's tumor by immunohistochemistry. Sample: Wilm's tumor. Primary antibody: Anti-Wilm's Tumor Protein Rabbit pAb (Cat. No. CA1026) (1:100). Detection: fluorescence.
References
ReferencesHuff, V. 1998. Am. J. Med. Genet. 79, 260.
Product Information
FormLiquid
FormulationIn 10 mM PBS, 0.2% BSA, pH 7.6.
Positive controlWilm's tumor tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Paraffin Sections
Application NotesImmunoblotting (see comments)
Paraffin Sections (1:100, heat pre-treatment required)
Immunoprecipitation (see comments)
Application CommentsStaining of formalin-fixed, paraffin-embedded tissues requires antigen retrieval by boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 min, followed by cooling at room temp for 20 min. This antibody is also reported to work for immunoblotting and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids from the C-terminus of human Wilm's tumor protein
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
CA1026 0

Documentation

Anti-Wilm's Tumor Protein Rabbit pAb MSDS

Title

Safety Data Sheet (SDS) 

Anti-Wilm's Tumor Protein Rabbit pAb Certificates of Analysis

TitleLot Number
CA1026

References

Reference overview
Huff, V. 1998. Am. J. Med. Genet. 79, 260.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-August-2007 RFH
SynonymsAnti-WT-1
ApplicationImmunoblotting (see comments)
Paraffin Sections (1:100, heat pre-treatment required)
Immunoprecipitation (see comments)
Application Data
Detection of Wilm's tumor by immunohistochemistry. Sample: Wilm's tumor. Primary antibody: Anti-Wilm's Tumor Protein Rabbit pAb (Cat. No. CA1026) (1:100). Detection: fluorescence.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~47-55 kDa Wilm's tumor protein.
BackgroundWilm's tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm's tumor and mesothelioma.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids from the C-terminus of human Wilm's tumor protein
IsotypeIgG
Specieshuman, mouse, rat
Positive controlWilm's tumor tissue
FormLiquid
FormulationIn 10 mM PBS, 0.2% BSA, pH 7.6.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsStaining of formalin-fixed, paraffin-embedded tissues requires antigen retrieval by boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 min, followed by cooling at room temp for 20 min. This antibody is also reported to work for immunoblotting and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage.
Toxicity Standard Handling
ReferencesHuff, V. 1998. Am. J. Med. Genet. 79, 260.