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ST1553 Anti-VCP Mouse mAb (4A8)

This Anti-VCP Mouse mAb (4A8) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of VCP.

Anti-VCP Mouse mAb (4A8) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Valosin-Containing Protein

ST1553
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
ST1553-100UG 100 μg
Description
OverviewRecognizes the ~67 kDa VCP protein in HePG2 cells and human placenta tissue.
Catalogue NumberST1553
Brand Family Calbiochem®
SynonymsAnti-Valosin-Containing Protein
Application Data
Detection of human VCP by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-VCP Mouse mAb (4A8) (Cat. No. ST1553) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human VCP by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human placenta tissue. Primary antibody: Anti-VCP Mouse mAb (4A8) (Cat. No. ST1553) (3 µg/ml). Detection: colorimetric (DAB).
References
Product Information
Formliquid
FormulationIn PBS, pH 7.2.
Positive controlHePG2 cells, human placenta tissue
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Paraffin Sections (3 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-length, recombinant human VCP (aa 1-589), expressed as a GST fusion protein
Clone4A8
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ST1553-100UG 04055977224276

Documentation

Anti-VCP Mouse mAb (4A8) MSDS

Title

Safety Data Sheet (SDS) 

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision08-November-2010 RFH
SynonymsAnti-Valosin-Containing Protein
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Paraffin Sections (3 µg/ml)
Application Data
Detection of human VCP by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-VCP Mouse mAb (4A8) (Cat. No. ST1553) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human VCP by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human placenta tissue. Primary antibody: Anti-VCP Mouse mAb (4A8) (Cat. No. ST1553) (3 µg/ml). Detection: colorimetric (DAB).
DescriptionPurified mouse monoclonal antibody. Recognizes the ~67 kDa VCP protein.
BackgroundVCP is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation.
HostMouse
ImmunogenFull-length, recombinant human VCP (aa 1-589), expressed as a GST fusion protein
Clone4A8
IsotypeIgG2a
Specieshuman
Positive controlHePG2 cells, human placenta tissue
Formliquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review