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MABN1777-100UG Anti-VAChT Antibody, clone N425/45

Anti-VAChT, clone N425/45, Cat. No. MABN1777, is a mouse monoclonal antibody that detects Vesicular acetylcholine transporter (VAChT) and is tested for use in Immunohistochemistry (Paraffin).

MABN1777-100UG
100 μg  
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Overview

Replacement Information
Description
Catalogue NumberMABN1777-100UG
DescriptionAnti-VAChT Antibody, clone N425/45
Alternate Names
  • Vesicular acetylcholine transporter
  • rVAT
  • Solute carrier family 18 member 3
Background InformationVesicular acetylcholine transporter (UniProt: Q62666; also known as VAChT, rVAT, Solute carrier family 18 member 3) is encoded by the Slc18a3 (also known as Vacht, Vat) gene (Gene ID: 60422) in rat. Acetylcholine (ACh) is synthesized in nerve terminals from choline and acetyl coenzyme A by the cytoplasmic choline acetyltransferase. ACh is then transported into synaptic vesicles where it is stored until release. This transport from cytoplasm into synaptic vesicles is achieved via vesicular acetylcholine transporter (VAChT) that acts as an antiporter to couple ACh entry into synaptic vesicles with proton efflux. VAChT is a mutli-pass membrane protein with seven cytoplasmic domains, twelve transmembrane domains, and six lumenal domains. High expression of VAChT is observed in all major cholinergic neurons, including postganglionic parasympathetic cells, preganglionic sympathetic and parasympathetic cells, ventral spinal cord, and brainstem motoneurons. It is weakly expressed in the cortex and hippocampus. Elevated levels of VAChT are reported to affect its own intraneuronal trafficking and can remodel striatal cholinergic interneuron morphology and reduce the number and increase the size of cholinergic varicosities. (Ref.: Arvidsson, U., et al. (1997). J. Comp. Neurol. 378(4); 454-467; Janickova, H., et al. (2017). J. Nurochem. 142(6); 857-875).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-VAChT, clone N425/45, Cat. No. MABN1777, is a mouse monoclonal antibody that detects Vesicular acetylcholine transporter (VAChT) and is tested for use in Immunohistochemistry (Paraffin).
Key Applications
  • Immunohistochemistry (Paraffin)
Biological Information
ImmunogenRecombinant fragment corresponding to the first lumenal domain of rat Vesicular acetylcholine transporter.
EpitopeN-terminal
CloneN425/45
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone N425/45 is a mouse monoclonal antibody that detects Vesicular acetylcholine transporter. It targets an epitope within the first lumenal region.
IsotypeIgG1κ
Species Reactivity
  • Rat
Species Reactivity NoteRat. Predicted to react with Mouse based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Slc18a3
  • Vacht
  • Vat
Purification MethodProtein G purified
UniProt Number
Molecular Weight56.54 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in rat brain tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected VAChT in rat brain tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN1777-100UG 04061841879695