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MAB1482-I Anti-Trypsin (Human pancreas) Antibody, clone TRYP11-236.7

Anti-Trypsin, human pancreas, clone TRYP11-236.7, Cat. No. MAB1482-I, is a mouse monoclonal antibody that detects Trypsin and is tested for use in and ELISA.

MAB1482-I
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMAB1482-I
DescriptionAnti-Trypsin (Human pancreas) Antibody, clone TRYP11-236.7
Alternate Names
  • Beta-trypsin
  • Cationic trypsinogen
  • Serine protease 1
  • Trypsin I
Background InformationTrypsin-1 (UniProt: P07477; also known as EC:3.4.21.4, Beta-trypsin, Cationic trypsinogen, Serine protease 1, Trypsin I) is encoded by the PRSS1 (also known as TRP1, TRY1, TRYP1) gene (Gene ID: 5644) in human. Trypsin is pancreatic serine protease that is synthesized as an inactive precursor known as trypsinogen that is processed through the Golgi apparatus and packaged into zymogen granules for secretion by exocytosis. Trypsin-1 is synthesized with a signal peptide (aa 1-15) and a propeptide (trypsin activation peptide; aa 16-23) that are subsequently cleaved off to generate the mature form that can be further cleaved and processed into alpha-trypsin chain 1 (aa 24-122) and alpha-trypsin chain 2 (aa 123-247). Trypsin can be present in a single-chain and a two-chain form, produced by proteolytic cleavage after arginine 122. Trypsin can also undergo sulfation at tyrosine 154 that increases its selectivity towards basic versus apolar residues at the P2' position of inhibitors that bind in a substrate-like fashion. Trypsin cleaves peptide chains mainly at the carboxyl side of the amino acid lysine or arginine, except when either is followed by proline.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal in buffer containing PBS, pH 7.4, containing no preservatives without preservatives.
Quality LevelMQ100
Applications
ApplicationAnti-Trypsin, human pancreas, clone TRYP11-236.7, Cat. No. MAB1482-I, is a mouse monoclonal antibody that detects Trypsin and is tested for use in and ELISA.
Key Applications
  • Immunohistochemistry (Paraffin)
  • ELISA
Application NotesImmunohistochestry/IH(P): {Solakidi, et al. 2003} 1:100-1:500; formalin fixed, paraffin embedded human tissues; 5 m serial sections; blocking solution 0.5% serum in PBS; overnight incubations; enzymatic detection.

ELISA: (Dilution is assay based)

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenPurified human pancreatic trypsin.
EpitopeUnknown
CloneTRYP11-236.7
ConcentrationPlease refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone TRYP11-236.7 is a mouse monoclonal antibody that detects human Trypsin-1.
IsotypeIgG1
Species Reactivity
  • Human
Species Reactivity NoteHuman
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PRSS1
  • TRP1
  • TRY1
  • TRYP1
Purification MethodProtein G purified
UniProt Number
Molecular Weight26.56 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20°C in convenient aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB1482-I 04065265651400

Documentation

Anti-Trypsin (Human pancreas) Antibody, clone TRYP11-236.7 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Trypsin (Human pancreas) Antibody, clone TRYP11-236.7 Certificates of Analysis

TitleLot Number
Anti-Trypsin (Human pancreas), clone TRYP11-236.7 - 3812534 3812534
Anti-Trypsin (Human pancreas), clone TRYP11-236.7 - 3821633 3821633
Anti-Trypsin (Human pancreas), clone TRYP11-236.7 - 3824801 3824801
Anti-Trypsin (Human pancreas), clone TRYP11-236.7 - 3826008 3826008
Anti-Trypsin (Human pancreas), clone TRYP11-236.7 - 3832604 3832604
Anti-Trypsin (Human pancreas), clone TRYP11-236.7 - 3833209 3833209
Anti-Trypsin (Human pancreas), clone TRYP11-236.7 - 3859743 3859743
Anti-Trypsin (Human pancreas), clone TRYP11-236.7 - 3862767 3862767
Anti-Trypsin (Human pancreas), clone TRYP11-236.7 - 3884784 3884784
Anti-Trypsin (Human pancreas), clone TRYP11-236.7 - 3898677 3898677
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