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MABT1336-25UL Anti-Tropomyosin 3 alpha chain Antibody, clone 4D10A5

Anti-Tropomyosin 3 alpha chain, clone 4D10A5, Cat. No. MABT1336, is a mouse monoclonal antibody that detects Tropomyosin alpha-3 chain and is tested for use in Western Blotting.

MABT1336-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABT1336-25UL
DescriptionAnti-Tropomyosin 3 alpha chain Antibody, clone 4D10A5
Alternate Names
  • Tropomyosin alpha-3 chain
  • Gamma-tropomyosin
  • Tropomyosin-3
  • Tropomyosin-5
  • Tpm3
Background InformationTropomyosin alpha-3 chain (UniProt: Q63610; also known as Gamma-tropomyosin, Tropomyosin-3, Tropomyosin-5, Tpm3) is encoded by the Tpm3 (also known as Tpm-5, Tpm5) gene (Gene ID: 117557) in rat. Tropomyosins are rod-like dimers of coiled-coil proteins that provide stability to actin filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Tropomyosin-3 is a homodimeric protein in slow-twitch skeletal muscle that can also form heterodimers of an alpha (TPM1, TPM3 or TPM4) and a beta (TPM2) chain. It binds to actin filaments in muscle and non-muscle cells and plays crucial role in the calcium dependent regulation of vertebrate striated muscle contraction. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Mutations in Tpm3 gene are associated with diseases of skeletal muscle such as nemaline myopathy, distal arthrogryposis, and the cap disease that is characterized by myopathy and hypotonia that progresses over time. Clone 4D10A5 recognizes the mammalian tropomyosin exon 9a present in muscle and cytoskeletal isoforms produced by the TPM1, TPM2 and TPM3 genes. (Ref.: Schevzov, G., et al. (2011). BioArchitecture, 1 (4); 135-164).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-Tropomyosin 3 alpha chain, clone 4D10A5, Cat. No. MABT1336, is a mouse monoclonal antibody that detects Tropomyosin alpha-3 chain and is tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected Tropomyosin 3 alpha chain in Western Blotting applications (Schevzov, G., et. al. (2011). Bioarchitecture. 1(4):135-164).

Western Blotting Analysis: A 1:125-500 dilution from a representative lot detected Tropomyosin 3 alpha chain in Mouse kidney, skeletal muscle, and recombinant 9a samples (Courtesy of Dr. Jeff Hook, Ph.D., School of Medical Sciences, UNSW Sydney Australia).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenDiphtheria Toxoid Carrier Protein conjugated linear peptide corresponding to 27 amino acids from the C-terminal region of isoform 2 of rat Tropomyosin alpha-3 chain (Tpm 3).
EpitopeC-terminal
Clone4D10A5
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 4D10A5 is a mouse monoclonal antibody that detects Tropomyosin alpha-3 (Tpm3) chain of isoform 2. It targets and epitope within 27 amino acids from the C-terminal region.
IsotypeIgG2bκ
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity NoteHuman, Mouse, Rat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Tpm3
  • Tpm-5
  • Tpm5
Purification MethodProtein G purified
UniProt Number
Molecular Weight28.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.



Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2b.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT1336-25UL 04061842378036

Documentation

Anti-Tropomyosin 3 alpha chain Antibody, clone 4D10A5 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Tropomyosin 3 alpha chain Antibody, clone 4D10A5 Certificates of Analysis

TitleLot Number
Anti-Tropomyosin 3 alpha chain, clone 4D10A5 - Q3484346 Q3484346

References

Reference overviewPub Med ID
Tropomyosin isoforms and reagents
Galina Schevzov 1 , Shane P Whittaker, Thomas Fath, Jim Jc Lin, Peter W Gunning
Bioarchitecture  1(4)  135-164  2010

Show Abstract 		22069507 22069507