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MABN2308-25UG Anti-TrkA Antibody, clone MNAC13

Anti-TrkA, clone MNAC13, Cat. No. MABN2308, is a mouse monoclonal antibody that detects TrkA and is tested for use in ELISA and Neutralizing.

MABN2308-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABN2308-25UG
DescriptionAnti-TrkA Antibody, clone MNAC13
Alternate Names
  • High affinity nerve growth factor receptor
  • EC:2.7.10.1
  • Neurotrophic tyrosine kinase receptor type 1
  • TRK1-transforming tyrosine kinase protein
  • Tropomyosin-related kinase A
  • Tyrosine kinase receptor
  • Tyrosine kinase receptor A
  • gp140trk
  • p140-TrkA
Background InformationHigh affinity nerve growth factor receptor (UniProt: P04629; also known as EC:2.7.10.1, Neurotrophic tyrosine kinase receptor type 1, TRK1-transforming tyrosine kinase protein, Tropomyosin-related kinase A, Tyrosine kinase receptor, Tyrosine kinase receptor A, Trk-A, gp140trk, p140-TrkA) is encoded by the NTRK1 (also known as MTC, TRK, TRKA) gene (Gene ID: 4914) in human. Trk-A is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-32), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 33-423), a transmembrane domain (aa 424-439), and a cytoplasmic domain (aa 440-796). The extracellular domain of Trk-A is composed by five domains: a leucine-rich region, two cysteine-rich domains and two immunoglobulin (Ig)-like domains. Trk-A serves as a receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous system through regulation of proliferation, differentiation, and survival of sympathetic and nervous neurons. It serves as a high affinity receptor for NGF, which is its primary ligand. It can also bind and be activated by neurotrophin-3 (NTF3). However, NTF3 only supports axonal extension through NTRK1 but does not affect neuron survival. Upon dimeric NGF ligand-binding, it undergoes homodimerization, autophosphorylation. and activation. Clone MNAC13 recognizes Trk-A with high affinity and specificity and displays a neutralizing activity toward the NGF/TrkA interaction. (Ref.: Covaceuszach, S., et al. (2005). Proteins. 58(3); :717-727; Jing, S., et al. (1992). Neuron. 9(6); 1067-1079).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in PBS without preservatives.
Quality LevelMQ200
Applications
ApplicationAnti-TrkA, clone MNAC13, Cat. No. MABN2308, is a mouse monoclonal antibody that detects TrkA and is tested for use in ELISA and Neutralizing.
Key Applications
  • ELISA
  • Neutralizing
Application NotesTested applications

ELISA Analysis: A representative lot detected TrkA in ELISA applications (Covaceuszach, S., et al. (2005). Proteins. 58(3):717-27).

Neutralizing: A representative lot neutralized TrkA activity in Neutralizing applications (Covaceuszach, S., et al. (2005). Proteins. 58(3):717-27; Ugolini, G., et al. (2007). Proc Natl Acad Sci USA. 104(8):2985-90).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenBALB/C 3T3-transfected cells expressing human Trk-A protein.
EpitopeExtracellular domain
CloneMNAC13
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone MNAC13 is a mouse monoclonal antibody that detects TrkA. It targets an epitope within 17 amino acids within the first Ig-like C2 type domain from the extracellular domain.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • NTRK1
Purification MethodProtein G purified
UniProt Number
Molecular Weight87.5 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by ELISA with recombinant Human TrkA.

ELISA Analysis (ELISA): Various dilutions of this antibody detected recombinant Human TrkA.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN2308-25UG 04065268900826