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MABT1514-25UG Anti-Tenascin-R Antibody, clone 23-14

Anti-Tenascin-R, clone 23-14, Cat. No. MABT1514, is a mouse monoclonal antibody that detects Tenascin-R and is tested for use in Immunohistochemistry (Paraffin), Inhibition Assay, and Western Blotting.

MABT1514-25UG
25 μg  
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Overview

Replacement Information
Description
Catalogue NumberMABT1514-25UG
DescriptionAnti-Tenascin-R Antibody, clone 23-14
Alternate Names
  • TN-R
  • Restrictin
Background InformationTenascin-R (UniProt: Q00546; also known as TN-R, Restrictin) is encoded by the TNR gene (Gene ID: 396213) in chicken. Tenascins are a family of four large glycoproteins (Tenascin-C, R, X, and W) that share a cysteine-rich N-terminus, a sequence of fibronectin type III domains, and homologies to fibronectin-b at the C-terminus. Tenascin-R (TN-R), the smallest member of the tenascin family that can exist as homodimer or homotrimer. It interacts with different cells and matrix components and is involved in neural cell adhesion and neurite formation. Its interaction with contactin-1 enhances neurite outgrowth. It is expressed weakly in E6 embryo and reaches a maximum at E16 and declines in the adult. Its expression is observed in oligodendrocytes during myelination, and it accumulates at Ranvier nodes and by a subpopulation of neurons, including cerebellar stellate/basket cells, motoneurons in the spinal cord, and hippocampal interneurons. TN-R is synthesized with a signal peptide (aa 1-33), which is subsequently cleaved off to generate the mature form. Its N-terminal region contains a cysteine-rich segment that it shares with other members of tenascin family. Within this segment there is a stretch of 20 amino acids that form three heptad repeats of hydrophobic amino acids in an a-helix that might generate a triple-stranded coiled coil to link three or two TN-R polypeptides to trimers or dimers. It also contains four EGF-like domains and nine fibronectin type-III (Fn-III) domains. Fn-III 9 mediates cell attachment and Fn-III 2-5 are reported to mediate neurofascin binding. It also contains a fibrinogen C-terminal domain (aa 1124-1339) that mediates its interaction with chondroitin Sulfate Proteoglycan 5. (Ref.: Nörenberg, U., et al. (1995). J. Cell Biol. 130(2); 473-484; Rathjen, FG., et al. (1991). Development.113(1); 151-164).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
Quality LevelMQ200
Applications
ApplicationAnti-Tenascin-R, clone 23-14, Cat. No. MABT1514, is a mouse monoclonal antibody that detects Tenascin-R and is tested for use in Immunohistochemistry (Paraffin), Inhibition Assay, and Western Blotting.
Key Applications
  • Western Blotting
  • Inhibition
  • Immunohistochemistry (Paraffin)
Application NotesTested Applications

Inhibition Assay: A representative lot of this antibody inhibited neural cell and retinal cell attachment to Tenascin-R. (Rathjen, F.G., et al. (1991). Development. 113(1):151-64; Norenberg, U., et al. (1995). J Cell Biol. 130(2):473-84; Norenberg, U., et al. (1996). Int J Dev Neurosci. 14(3):217-31).

Immunohistochemistry (Paraffin) Analysis: A 1:50-250 dilution from a representative lot detected Tenascin-R in mouse brain and rat brain tissue sections.

Western Blotting Analysis: A representative lot detected Tenascin-R in Western Blotting applications (Wintergerst, E.S., et al. (2001). J Neurocytol. 30(4):293-301).

Immunohistochemistry Analysis: A representative lot detected Tenascin-R in Immunohistochemistry applications (Haunso, A., et al. (2000). Brain Res.;864(1):142-5; Wintergerst, E.S., et al. (2001). J Neurocytol.;30(4):293-301; Geissler, M., et al. (2013). J Neurosci.;33(18):7742-55).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenImmunoaffinity isolate of the monoclonal antibody F11 from detergent extracts of adult chicken brain plasma membrane preparations.
EpitopeC-terminal half
Clone23-14
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 23-14 is a mouse monoclonal antibody that detects Tenascin-R. It targets an epitope within the Fibronectin type-III 8 domain from the C-terminal half.
IsotypeIgG1κ
Species Reactivity
  • Rat
  • Chicken
  • Mouse
  • Human
Species Reactivity NoteHuman, Chicken, Mouse, Rat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • TNR
Purification MethodProtein G purified
UniProt Number
Molecular Weight~170 kDa observed; 148.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Rat brain tissue lysates.

Western Blotting Analysis: A 1:125 dilution of this antibody detected Tenascin-R in Rat brain tissue lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT1514-25UG 04065267780641