Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

582000 Anti-Telomerase (Ab-1) Rabbit pAb

This Anti-Telomerase (Ab-1) Rabbit pAb is validated for use in Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of Telomerase (Ab-1).

Anti-Telomerase (Ab-1) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-TERT-1

582000
  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, MRbPolyclonal Antibody
Description
OverviewRecognizes the ~125 kDa telomerase (TERT) protein.
Catalogue Number582000
Brand Family Calbiochem®
SynonymsAnti-TERT-1
References
ReferencesFu, W., et al. 2000. J. Mol. Neurosci. 14, 3.
Kharbanda, S., et al. 2000. Curr. Biol. 10, 568.
Vaziri, H., et al. 1999. Mol. Cell. Biol. 19, 2373.
Product Information
FormLiquid
FormulationUndiluted serum.
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Paraffin Sections
Application NotesImmunoblotting (1:100, see comments)
Immunoprecipitation (1:40, see comments)
Paraffin Sections (1:250-1:1000, see comments)
Application CommentsAntibody works only weakly in paraffin-embedded tissue sections. Nuclear extracts are recommended for immunoblotting and immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogena synthetic peptide corresponding to amino acids from an internal region of the catalytic subunit of human telomerase reverse transcriptase (hTERT)
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
582000 0

Documentation

Anti-Telomerase (Ab-1) Rabbit pAb MSDS

Title

Safety Data Sheet (SDS) 

Anti-Telomerase (Ab-1) Rabbit pAb Certificates of Analysis

TitleLot Number
582000

References

Reference overview
Fu, W., et al. 2000. J. Mol. Neurosci. 14, 3.
Kharbanda, S., et al. 2000. Curr. Biol. 10, 568.
Vaziri, H., et al. 1999. Mol. Cell. Biol. 19, 2373.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision25-September-2007 JSW
SynonymsAnti-TERT-1
ApplicationImmunoblotting (1:100, see comments)
Immunoprecipitation (1:40, see comments)
Paraffin Sections (1:250-1:1000, see comments)
DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~125 kDa telomerase (TERT) protein.
BackgroundThe 3' ends of chromosomes are capped with telomere sequences (TTAGGG; 6-26 nucleotides in length) by ribonucleo-protein telomerase during DNA replication. Telomerase is an unusual RNA-dependent DNA polymerase that uses an RNA component to specify the addition of telomere. In ciliated protozoa and yeast, telomere length is maintained by regulating the activity of telomerase. Many mammalian cells do not express telomerase, resulting into shortening of the telomere with each cell division, ultimately causing chromosomal instability, aging and cell death. ~4.8 kb of telomeric DNA is lost with each cell division. Most recently, introduction of telomerase into normal human cells has been shown to extend normal cell life by ~20 doublings.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids from an internal region of the catalytic subunit of human telomerase reverse transcriptase (hTERT)
IsotypeIgG
Specieshuman, mouse
FormLiquid
FormulationUndiluted serum.
PreservativeNone
CommentsAntibody works only weakly in paraffin-embedded tissue sections. Nuclear extracts are recommended for immunoblotting and immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesFu, W., et al. 2000. J. Mol. Neurosci. 14, 3.
Kharbanda, S., et al. 2000. Curr. Biol. 10, 568.
Vaziri, H., et al. 1999. Mol. Cell. Biol. 19, 2373.