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CB1052 Anti-TUBB2A Mouse mAb (2B2)

This Anti-TUBB2A Mouse mAb (2B2) is validated for use in Immunoblotting, Paraffin Sections for the detection of TUBB2A.

Anti-TUBB2A Mouse mAb (2B2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Tubulin β2A

CB1052
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
CB1052-100UG 100 μg
Description
OverviewRecognizes the ~48-50 kDa TUBB2A protein in PC-12, Raw 264.7, Jurkat, and NIH3T3 cells and human spleen tissue.
Catalogue NumberCB1052
Brand Family Calbiochem®
SynonymsAnti-Tubulin β2A
Application Data
Detection of rat TUBB2A by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-TUBB2A Mouse mAb (2B2) (Cat. No. CB1052) (1-5 µg/ml). Detection: chemiluminescence.

Detection of mouse TUBB2A by immunoblotting. Sample: Whole cell lysate from Raw 264.7 cells. Primary antibody: Anti-TUBB2A Mouse mAb (2B2) (Cat. No. CB1052) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human TUBB2A by immunoblotting. Sample: Whole cell lysate from Jurkat cells. Primary antibody: Anti-TUBB2A Mouse mAb (2B2) (Cat. No. CB1052) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human TUBB2A by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human spleen tissue. Primary antibody: Anti-TUBB2A Mouse mAb (2B2) (Cat. No. CB1052) (1.5 µg/ml). Detection: colorimetric (DAB).
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Negative control293T cells
Positive controlPC-12 cells, Raw 264.7 cells, Jurkat cells, NIH3T3 cells, Human spleen tissue
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesImmunoblotting (1-5 µg/ml)
Paraffin Sections (1.5 µg/ml)
Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-length, recombinant, human TUBB2A (aa 1-446), expressed as a GST fusion protein
Clone2B2
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
CB1052-100UG 04055977228458

Documentation

Anti-TUBB2A Mouse mAb (2B2) MSDS

Title

Safety Data Sheet (SDS) 

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision01-November-2010 RFH
SynonymsAnti-Tubulin β2A
ApplicationImmunoblotting (1-5 µg/ml)
Paraffin Sections (1.5 µg/ml)
Application Data
Detection of rat TUBB2A by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-TUBB2A Mouse mAb (2B2) (Cat. No. CB1052) (1-5 µg/ml). Detection: chemiluminescence.

Detection of mouse TUBB2A by immunoblotting. Sample: Whole cell lysate from Raw 264.7 cells. Primary antibody: Anti-TUBB2A Mouse mAb (2B2) (Cat. No. CB1052) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human TUBB2A by immunoblotting. Sample: Whole cell lysate from Jurkat cells. Primary antibody: Anti-TUBB2A Mouse mAb (2B2) (Cat. No. CB1052) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human TUBB2A by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human spleen tissue. Primary antibody: Anti-TUBB2A Mouse mAb (2B2) (Cat. No. CB1052) (1.5 µg/ml). Detection: colorimetric (DAB).
DescriptionPurified mouse monoclonal antibody. Recognizes the ~48-50 kDa TUBB2A protein.
BackgroundTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the β-chain and one at a non-exchangeable site on the α-chain.
HostMouse
ImmunogenFull-length, recombinant, human TUBB2A (aa 1-446), expressed as a GST fusion protein
Clone2B2
IsotypeIgG2a
Specieshuman, mouse, rat
Positive controlPC-12 cells, Raw 264.7 cells, Jurkat cells, NIH3T3 cells, Human spleen tissue
Negative control293T cells
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsVariables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review