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AP1163 Anti-TP53BP2 Mouse mAb (3F8)

This Anti-TP53BP2 Mouse mAb (3F8) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of TP53BP2.

Anti-TP53BP2 Mouse mAb (3F8) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Tumor Protein p53 Binding Protein 2

AP1163
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody
Description
OverviewRecognizes the ~120 kDa TP53BP2 protein in HepG2 cells and human testes tissue.
Catalogue NumberAP1163
Brand Family Calbiochem®
SynonymsAnti-Tumor Protein p53 Binding Protein 2
Application Data
Detection of human TP53BP2 by iummunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-TP53BP2 Mouse mAb (3F8) (Cat. No. AP1163) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human TP53BP2 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human testes tissue. Primary antibody: Anti-TP53BP2 Mouse mAb (3F8) (Cat. No. AP1163) (3 µg/ml). Detection: colorimetric (DAB).
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Positive controlHepG2 cells, Human testes tissue
PreservativeNone
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Paraffin Sections (3 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids of 511-610 human TP53BP2, expressed as a GST fusion protein
Clone3F8
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AP1163 0

Documentation

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision19-October-2010 RFH
SynonymsAnti-Tumor Protein p53 Binding Protein 2
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Paraffin Sections (3 µg/ml)
Application Data
Detection of human TP53BP2 by iummunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-TP53BP2 Mouse mAb (3F8) (Cat. No. AP1163) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human TP53BP2 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human testes tissue. Primary antibody: Anti-TP53BP2 Mouse mAb (3F8) (Cat. No. AP1163) (3 µg/ml). Detection: colorimetric (DAB).
DescriptionPurified mouse monoclonal antibody. Recognizes the ~120 kDa TP53BP2 protein.
BackgroundTP53BP2 is a member of the ASPP (apoptosis-stimulating protein of p53) family of p53 interacting proteins. The protein contains four ankyrin repeats and an SH3 domain involved in protein-protein interactions. It is localized to the perinuclear region of the cytoplasm, and regulates apoptosis and cell growth through interactions with other regulatory molecules including members of the p53 family.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids of 511-610 human TP53BP2, expressed as a GST fusion protein
Clone3F8
IsotypeIgG2a
Specieshuman
Positive controlHepG2 cells, Human testes tissue
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review