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MAB3216-100ug Anti-TNF Receptor Antibody, extracellular, clone H398

Detect TNF Receptor using this Anti-TNF Receptor Antibody, extracellular, clone H398 validated for use in ELISA, FC, IP, RIA & WB.

Research Category: Inflammation & Immunology Research Sub-Category: Cytokines & Cytokine Receptors Gene Symbol: TNFRSF1A, TNFR1, TNF-R1, TNFR-I, p60, CD120a, p55-R, TNF-RI, TNFR60, TNF-R55, TNFAR, TBP1, TNFR55, TNF-R-I, FPF, TNF-R, p55, MGC19588 UniProt Number: P19438
MAB3216-100ug
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HELISA, FC, IP, RIA, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB3216-100ug
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-TNF Receptor Antibody, extracellular, clone H398
Alternate Names
  • CD120a
  • TNF-R1
  • p55
Background InformationTumor necrosis factor receptor superfamily member 1A (TNF-receptor 1 or CD120a) is a receptor for TNFSF2/TNF-alpha. TNF receptor mediates apoptosis and contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. Binding of TNF to the extracellular domain of TNF receptor leads to homotrimerization. Defects in TNF receptor cause tumor necrosis factor receptor-associated periodic syndrome (TRAPS), a hereditary periodic fever syndrome characterized by recurrent fever, abdominal pain, localized tender skin lesions and myalgia.
References
Product Information
FormatPurified
PresentationIgG fraction purified from tissue culture supernatant by protein-A affinity chromatography. Liquid in PBS pH 7.4 containing 0.09% sodium azide.
Applications
ApplicationDetect TNF Receptor using this Anti-TNF Receptor Antibody, extracellular, clone H398 validated for use in ELISA, FC, IP, RIA & WB.
Key Applications
  • ELISA
  • Flow Cytometry
  • Immunoprecipitation
  • Radioimmunoassay
  • Western Blotting
Application NotesELISA

Flow Cytometry

Western Blotting: non reducing conditions only

Immunoprecipitation

Radioimmunoassay

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenRecombinant human tumor necrosis factor receptor type 1
EpitopeExtracellular Domain
CloneH398
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityRecognizes an extracellular domain of the 55 kDa human TNF receptor (p55, TNF-R1, CD120a). No binding occurs to the 75 kDa TNF receptor (CD120b). This antibody inhibits the biological activity of both natural and recombinant human TNFa and TNFbthrough competitive inhibition of TNF binding to TNF-R1. MAB3216-100ug shows potent antagonistic activity in in vitro TNF mediated cytotoxicity assays (IC50=12nM; IC50=1.7 mg/mL).
IsotypeIgG2a
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is one of the major receptors for the tumor necrosis factor-alpha. This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor. Germline mutations of the extracellular domains of this receptor were found to be associated with the autosomal dominant periodic fever syndrome. The impaired receptor clearance is thought to be a mechanism of the disease.
Gene Symbol
  • TNFRSF1A
  • TNFR1
  • TNF-R1
  • TNFR-I
  • p60
  • CD120a
  • p55-R
  • TNF-RI
  • TNFR60
  • TNF-R55
  • TNFAR
  • TBP1
  • TNFR55
  • TNF-R-I
  • FPF
  • TNF-R
  • p55
  • MGC19588
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P19438 # Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate- specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
SIZE: 455 amino acids; 50495 Da
SUBUNIT: Binding of TNF to the extracellular domain leads to homotrimerization. The aggregated death domains provide a novel molecular interface that interacts specifically with the death domain of TRADD. Various TRADD-interacting proteins such as TRAFS, RIPK1 and possibly FADD, are recruited to the complex by their association with TRADD. This complex activates at least two distinct signaling cascades, apoptosis and NF-kappa-B signaling. Interacts with BAG4, BRE, GRB2, SQSTM1 and TRPC4AP. Interacts with HCV core protein.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Secreted.
DOMAIN: SwissProt: P19438 The domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE. & Both the cytoplasmic membrane-proximal region and the C- terminal region containing the death domain are involved in the interaction with TRPC4AP (By similarity).
PTM: The soluble form is produced from the membrane form by proteolytic processing.
DISEASE: "SwissProt: P19438 # Defects in TNFRSF1A are the cause of familial hibernian fever (FHF) [MIM:142680]; also known as tumor necrosis factor receptor-associated periodic syndrome (TRAPS). FHF is an autosomal dominant disease characterized by recurrent fever, abdominal pain, localized tender skin lesions and myalgia."
SIMILARITY: Contains 1 death domain. & Contains 4 TNFR-Cys repeats.
Molecular Weight~50 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at 2-8°C in undiluted aliquots for short term or -20°C up to one year.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB3216-100ug 0

Documentation