Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

09-403 Anti-TKS5 (SH3 #1) Antibody

Anti-TKS5 (SH3 #1) Antibody detects level of TKS5 (SH3 #1) & has been published & validated for use in WB, IC & IP.

Anti-TKS5 (SH3 #1) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeletal Signaling Gene Symbol: FISH , KIAA0418 , SH3MD1 , Sh3pxd2a, MGC144076 UniProt Number: O89032
09-403
100 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, ICC, IPRbPurifiedPolyclonal Antibody
Description
Catalogue Number09-403
DescriptionAnti-TKS5 (SH3 #1) Antibody
Alternate Names
  • Adaptor protein TKS5
  • Five SH3 domain-containing protein
  • SH3 and PX domains 2A
  • SH3 multiple domains 1
  • SH3 multiple domains protein 1
  • five SH3 domains
Background InformationTks5 (previously known as Fish), is a large scaffolding protein with one amino-terminal PX domain and five SH3 domains. It is cytoplasmic in normal fibroblasts. In Src-transformed cells, Tks5 localizes to podosomes. The coexpression of Tks5 and constitutively active Src in epithelial cancer cells resulted in the appearance of podosomes/invadopodia. Cells with reduced Tks5 expression are poorly invasive through matrigel. Tks5 is expressed and localized to podosomes/invadopodia in some invasive human cancer cell lines and tumor tissue, particularly breast cancers and melanomas. Thus, Tks5 appears to be required for podosome/invadopodia formation and for invasion of some cancer cells.
References
Product Information
FormatPurified
HS Code3002 15 90
Control
  • Western Blot::
    NIH-3T3 (100% confluent) lysate
    Immunocytochemistry:
    Mouse 3T3-Src(Y527F) cells
PresentationPurified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.
Quality LevelMQ100
Applications
ApplicationAnti-TKS5 (SH3 #1) Antibody detects level of TKS5 (SH3 #1) & has been published & validated for use in WB, IC & IP.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunoprecipitation
Application NotesWestern Blot Analysis: Antibody detected TKS5 in 20 μg of NIH-3T3 (100% confluent) lysate.
Biological Information
ImmunogenRecombinant Protein, aa: 166-225. Homology: Human, Dog, Horse, Rat, Cow (100%, 60/60); Chicken (93%, 56/60)
Epitope166-225 aa
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteReacts with Mouse and Human. Other homologies: Human, Canine, Equine, Rat, and Bovine (100% sequence homology), Chicken (93% sequence homology)
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • FISH
  • KIAA0418
  • SH3MD1
  • Sh3pxd2a
  • MGC144076
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFunction: Required for podosome formation, degradation of the extracellular matrix, and for the invasiveness of some cancer cells. Binds phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 3,4-biphosphate (PtdIns(3,4)P2). In association with ADAM12, mediates the neurotoxic effect of beta-amyloid peptide By similarity.

Subunit: structure Interacts with ADAM12, ADAM15 and ADAM19 By similarity.

Subcellular location: Cytoplasm. Cell projection › podosomeBy similarity. Note= Cytoplasmic in normal cells and localizes to podosomes in Src-transformed cells By similarity.

Tissue specificity: Widely expressed. Not found in the spleen and testis. Ref.1

Domain: The PX domain is required for podosome localization, and for binding phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 3,4-biphosphate (PtdIns(3,4)P2) By similarity.

The fifth SH3 domain mediates binding with ADAM12, ADAM15 and ADAM19 By similarity.

Post-translational modification: Tyrosine phosphorylated by SRC. Phosphorylation plays a regulatory role in the protein localization. The intramolecular interaction of the PX domain with the third SH3 domain maintains the protein in the cytoplasm and phosphorylation disrupts this interaction, resulting in the redistribution of the protein from cytoplasm to the perimembrane region. Phosphorylated on serine upon DNA damage, probably by ATM or ATR By similarity.

Sequence similarities: Belongs to the SH3PXD2 family.
Contains 1 PX (phox homology) domain.
Contains 5 SH3 domains.
Molecular Weight130-150 kDa. Uncharacterized bands(s) may be observed in some cell lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western Blot on NIH-3T3 (100% confluent) lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
09-403 04053252620867

Documentation

Anti-TKS5 (SH3 #1) Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-TKS5 (SH3 #1) Antibody Certificates of Analysis

TitleLot Number
Anti-TKS5 (SH3 #1) - 2398908 2398908
Anti-TKS5 (SH3 #1) - 2051967 2051967
Anti-TKS5 (SH3 #1) - 2199713 2199713
Anti-TKS5 (SH3 #1) - 2299968 2299968
Anti-TKS5 (SH3 #1) - 2510313 2510313
Anti-TKS5 (SH3 #1) - 2827145 2827145
Anti-TKS5 (SH3 #1) - 2930203 2930203
Anti-TKS5 (SH3 #1) - 3174822 3174822
Anti-TKS5 (SH3 #1) - 3611143 3611143
Anti-TKS5 (SH3 #1) - 3782274 3782274
Show more

Technical Info

Title
Fluorescent Gelatin Degradation Assays for Investigating Invadopodia Formation