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04-1525 Anti-TAFII250 Antibody, clone 6B3

Anti-TAFII250 Antibody, clone 6B3 is a Mouse Monoclonal Antibody for detection of TAFII250 also known as Cell cycle gene 1 protein & has been validated in WB, ICC, IHC & IP.

Anti-TAFII250 Antibody, clone 6B3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: TAF1, BA2R, CCG1, CCGS, DYT3, KAT4, N-TAF1, NSCL2, OF, P250, TAF(II)250, TAF2A, TAFII-250, TAFII250, p250 UniProt Number: P21675
04-1525
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICC, IHC, IPMPurifiedMonoclonal Antibody
Description
Catalogue Number04-1525
DescriptionAnti-TAFII250 Antibody, clone 6B3
Alternate Names
  • Cell cycle gene 1 protein
  • TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa
  • TAF1 RNA polymerase II, TATA box binding protein-associated factor, 250kDa
  • TATA box binding protein (TBP)-associated factor, RNA polymerase II, A, 250kD
  • TBP-associated factor 1
  • TBP-associated factor 250 kDa
  • Transcription initiation factor TFIID 250 kDa subunit
  • cell cycle, G1 phase defect
  • complementation of cell cycle block, G1-to-S 2
    transcription factor TFIID p250 polypeptide
Background InformationTATA-binding protein associated factor 250 (TAFII250) is the largest subunit of the transcription factor TFIID. Studies have shown that TAFII250 has serine/threonine domains that can autophosphorylate and transphosphorylate the large subunit of TFIIF. More recent studies have shown that TAFII250 is also a histone acetyltransferase, suggesting that transcriptional co-activators may also have a role in targeting acetylation activity to promoters.
References
Product Information
FormatPurified
Control
  • HeLa cell lysate
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-TAFII250 Antibody, clone 6B3 is a Mouse Monoclonal Antibody for detection of TAFII250 also known as Cell cycle gene 1 protein & has been validated in WB, ICC, IHC & IP.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunoprecipitation
Application NotesImmunoprecipitation
Cited by external research group using a representative lot. See Hilton, H.L., et. al (2005) Mol. Cell. Bio. 25(10):4321–4332.
Biological Information
ImmunogenHuman TAFII250
EpitopeUnknown
Clone6B3
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes TAFII250.
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryInitiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. This gene is part of a complex transcriptional unit (TAF1/DYT3), wherein some products share exons with TAF1 as well as additional exons downstream.[provided by RefSeq].
Gene Symbol
  • TAF1
  • BA2R
  • CCG1
  • CCGS
  • DYT3
  • KAT4
  • N-TAF1
  • NSCL2
  • OF
  • P250
  • TAF(II)250
  • TAF2A
  • TAFII-250
  • TAFII250
  • p250
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: Largest component and core scaffold of the TFIID basal transcription factor complex. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity. Essential for progression of the G1 phase of the cell cycle.

CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.

COFACTOR: Magnesium.

ENZYME REGULATION: Autophosphorylates on Ser residues. Inhibited by retinoblastoma tumor suppressor protein, RB1.

SUBUNIT STRUCTURE: TAF1 is the largest component of transcription factor TFIID that is composed of TBP and a variety of TBP-associated factors. TAF1, when part of the TFIID complex, interacts with C-terminus of TP53. RB1 interacts with the N-terminal domain of TAF1. Interacts with ASF1A and ASF1B. Interacts with SV40 Large T antigen.

SUBCELLULAR LOCATION: Nucleus

PTM: Phosphorylated by casein kinase II in vitro.

INVOLVEMENT IN DISEASE: Defects in TAF1 are the cause of dystonia type 3 (DYT3) [MIM:314250]; also called X-linked dystonia-parkinsonism (XDP). DYT3 is a X-linked dystonia-parkinsonism disorder. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT3 is characterized by severe progressive torsion dystonia followed by parkinsonism. Its prevalence is high in the Philippines. DYT3 has a well-defined pathology of extensive neuronal loss and mosaic gliosis in the striatum (caudate nucleus and putamen) which appears to resemble that in Huntington disease.

SEQUENCE SIMILARITIES: Belongs to the TAF1 family.

Contains 2 bromo domains.

Contains 1 HMG box DNA-binding domain.

Contains 2 protein kinase domains.
Molecular WeightCalculated molecular weight is 212 kDa. However protein is usually observed at 250 kDa band.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1 µg/ml of this antibody detected TAFII250 on 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
04-1525 04053252511264

Documentation

Anti-TAFII250 Antibody, clone 6B3 MSDS

Title

Safety Data Sheet (SDS) 

Anti-TAFII250 Antibody, clone 6B3 Certificates of Analysis

TitleLot Number
Anti-TAFII250, clone 6B3 3051262
Anti-TAFII250, clone 6B3 2485682
Anti-TAFII250, clone 6B3 2832935
Anti-TAFII250, clone 6B3 - 2202493 2202493
Anti-TAFII250, clone 6B3 - 2536530 2536530
Anti-TAFII250, clone 6B3 - 3426790 3426790
Anti-TAFII250, clone 6B3 - NG1917457 NG1917457
Anti-TAFII250, clone 6B3 - NRG1687966 NRG1687966
Anti-TAFII250, clone 6B3 -2669848 2669848
Anti-TAFII250, clone 6B3 -2813507 2813507
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