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ABN368 Anti-Spastin Antibody

Detect Spastin using this rabbit polyclonal antibody, Anti-Spastin Antibody validated for use in western blotting & ICC.

Anti-Spastin Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Signaling Gene Symbol: SPAST, ADPSP, FSP2, KIAA1083, SPG4 UniProt Number: Q9UBP0
ABN368
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, ICCRbSerumPolyclonal Antibody
Description
Catalogue NumberABN368
DescriptionAnti-Spastin Antibody
Alternate Names
  • Spastin
  • Spastic paraplegia 4 protein
Background InformationSpastin is a distinct microtubule-severing protein. Autosomal dominant mutations in the SPG4 locus corresponding to spastin are the most common cause of hereditary spastic paraplegia (HSP), a neurodegenerative disease that afflicts the adult corticospinal tracts.
References
Product Information
FormatSerum
Control
  • L6 cell lysate
PresentationRabbit polyclonal serum containing 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Spastin using this rabbit polyclonal antibody, Anti-Spastin Antibody validated for use in western blotting & ICC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: A representative lot from an independent laboratory detected Spastin in myc-Spastin transfected HeLa and MRC5 cell lysates (Connell, et al. (2009). Traffic. 10:42-56).
Biological Information
ImmunogenRecombinant protein corresponding to human Spastin with 4 isoforms.
HostRabbit
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • SPAST
  • ADPSP
  • FSP2
  • KIAA1083
  • SPG4
Purification MethodUnpurified
UniProt Number
Molecular Weight~ 68, 64, 58, 55 kDa observed. Uniprot describes 4 isoforms produced by alternative splicing at ~68 kDa, ~64 kDa, ~58 kDa, and ~55 kDa. Spastin has shown in some western blots that four isoforms [full length (68 kD), exon 4-deleted full length (64 kD), short form (58-60 kD) and exon 4-deleted short form (55 kD)] are expressed in mammalian cells. Ths pAb detects bands corresponding to the size of these four isoforms in HeLa, MRC5 human lung fibroblast and NSC34 mouse lower motor neuron neuroblastoma fusion cell lines (Connell, et al. (2009). Traffic. 10:42-56).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in L6 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Spastin in 10 µg of L6 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABN368 04053252909641