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MABN790 Anti-Shank3 Antibody, clone 16E2.3

This Anti-Shank3 Antibody, clone 16E2.3 is validated for use in Western Blotting for the detection of Shank3.

Anti-Shank3 Antibody, clone 16E2.3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Signaling Gene Symbol: SHANK3, KIAA1650, PROSAP2, PSAP2 UniProt Number: Q9BYB0
MABN790
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN790
DescriptionAnti-Shank3 Antibody, clone 16E2.3
Alternate Names
  • SH3 and multiple ankyrin repeat domains protein 3
  • Shank3
  • Proline-rich synapse-associated protein 2
  • ProSAP2
  • Shank3
Background InformationSH3 and multiple ankyrin repeat domains protein 3 (UniProt Q9BYB0; also known as Proline rich synapse associated protein 2, ProSAP2, Shank postsynaptic density protein, Shank3) is encoded by the SHANK3 (also known as DEL22q13.3, PROSAP2, PSAP2, SCZD15, SPANK-2) gene (Gene ID 85358) in human. Shank3/ProSAP2 belongs to the ProSAP/Shank family of scaffolding proteins, which are important dendritic spines postsynaptic density (PSD) complex components that play an important role in linking membrane glutamate receptors to intracellular signaling proteins and actin cytoskeleton. Shank proteins interact with adaptor proteins and assembles glutamate receptors at the postsynaptic membrane via multiple domains, including ankyrin repeat, proline-rich, SH3, PSD95/disc-large/zona occludens-1 (PDZ), and SAM domains. Shanks, together with the adaptor protein Homer and the endocytic vesicle-associated protein dynamin-3, are required for proper localization of the endocytic zone in close proximity to the PSD. Shank-3 induces formation of functional dendritic spines in aspiny cerebellar neurons, whereas Shank-3 knockdown results in reduced spine density and size in cultured hippocampal neurons. SHANK3 gene mutations are known causes of mental retardation and autism spectrum disorders.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Shank3 Antibody, clone 16E2.3 is validated for use in Western Blotting for the detection of Shank3.
Key Applications
  • Western Blotting
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to the C-terminal half of human Shank3.
EpitopeInternal (C-terminal half)
Clone16E2.3
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityExpected to react with both isoform 1 (A) and 2 (B).
IsotypeIgG2aκ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse. Predicted to react with Human based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SHANK3
  • KIAA1650
  • PROSAP2
  • PSAP2
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~185 kDa observed. Multiplet banding pattern as a result of isoforms and/or posttranslational modifications. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected Shank3 in 10 µg of mouse brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN790 04055977274950