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05-731-I-25UG Anti-SH3KBPI Antibody, clone 179.1.E1

Anti-SH3KBPI, clone 179.1.E1, Cat. No. 05-731-I, is a mouse monoclonal antibody that detects SH3 domain-containing kinase-binding protein 1 and has tested for use in Western Blotting.

05-731-I-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number05-731-I-25UG
DescriptionAnti-SH3KBPI Antibody, clone 179.1.E1
Alternate Names
  • SH3 domain-containing kinase-binding protein 1
  • Regulator of ubiquitous kinase
  • Ruk
  • SH3-containing expressed in tumorigenic astrocytes
Background InformationSH3 domain-containing kinase-binding protein 1 (UniProt: Q925Q9; also known as Regulator of ubiquitous kinase, Ruk, SH3-containing expressed in tumorigenic astrocytes, SH3KBPI,) is encoded by the Sh3kbp1 (also known as Ruk, Seta) gene (Gene ID: 84357) in rat. SH3KBPI is a peripheral membrane protein that serves as an adapter protein involved in regulating diverse signal transduction pathways. It localizes in endocytic vesicles containing clustered receptors. It is shown to be highly expressed in the spinal cord. It can self-associate and form homotetramers. It contains three SH3 domains (aa 1-58; 98-157; and 311-372). SH3KBPI is involved in the regulation of endocytosis and lysosomal degradation of ligand-induced receptor tyrosine kinases, including EGFR and MET/hepatocyte growth factor receptor, through an association with CBL and endophilins. It can attenuate phosphatidylinositol 3-kinase activity by interaction with its regulatory subunit. It is also reported to plays a role in the regulation of cell morphology and cytoskeletal organization. Seven different isoforms of SH3KBP1 have been described that are produced by alternative splicing.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-SH3KBPI, clone 179.1.E1, Cat. No. 05-731-I, is a mouse monoclonal antibody that detects SH3 domain-containing kinase-binding protein 1 and has tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot from this antibody detected SH3KBPI in NG108-15 cell lysate.
Biological Information
ImmunogenHis-tagged full-length recombinant rat SH3 domain-containing kinase-binding protein 1.
Clone179.1.E1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 179.1.E1 is a mouse monoclonal antibody that specifically detects SH3 domain-containing kinase-binding protein 1.
IsotypeIgG2bκ
Species Reactivity
  • Rat
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse, Rat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Sh3kbp1
  • Ruk
  • Seta
Purification MethodProtein G purified
UniProt Number
Molecular Weight~85 kDa observed; 78.09 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in THP-1 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected SH3KBPI in THP-1 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
05-731-I-25UG 04054839757815