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MABS1708 Anti-SAV1 Antibody, clone 3A1.1

Anti-SAV1, clone 3A1.1, Cat. No. MABS1708, is a mouse monoclonal antibody that detects Protein salvador homolog 1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

MABS1708
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABS1708
DescriptionAnti-SAV1 Antibody, clone 3A1.1
Alternate Names
  • Protein salvador homolog 1
  • 45 kDa WW domain protein
  • hWW45
Background InformationProtein salvador homolog 1 (UniProt: Q9H4B6; also known as 45 kDa WW domain protein, hWW45) is encoded by the SAV1 (also known as WW45) gene (Gene ID: 60485) in human. SAV1, a homodimeric protein is ubiquitously expressed in adult tissues with highest expression observed in the pancreas, aorta, and interventricular septum and lowest expression in skeletal muscle. SAV1 protein contains two WW domains (aa 199-232 and 234-267) and one SARAH domain (aa 321-368). SAV1 is phosphorylated by STK3/MST2 and STK4/MST1, but phosphorylation is not required for its stability. However, it increases the number of protein binding sites on the scaffold molecule. SAV1 is also required for STK3/MST2 and STK4/MST1 activation and promotes cell-cycle exit and terminal differentiation in developing epithelial tissues. SAV1 regulates their activity in the Hippo signaling pathway, which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. STK3/MST2 and STK4/MST1- SAV1 complex is also shown to phosphorylate and activate LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 inhibits its translocation into the nucleus.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-SAV1, clone 3A1.1, Cat. No. MABS1708, is a mouse monoclonal antibody that detects Protein salvador homolog 1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected SAV1 in 10 µg of OVCAR3 cell lysate.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected SAV1 in human kidney and human pancreas tissues.
Biological Information
ImmunogenGST/His-tagged recombinant fragment corresponding to 101 amino acids from the C-terminal half of human Protein salvador homolog 1 (SAV1).
Clone3A1.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 3A1.1 detects Protein salvador homolog 1 in human cells It targets an epitope with in 101 amino acids from the C-terminal half.
IsotypeIgG2aκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SAV1
  • WW45
Purification MethodProtein G purified
UniProt Number
Molecular Weight~45 kDa observed; 44.63 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HCT116 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected SAV1 in 10 µg of HCT116 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS1708 04054839316012

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