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MABN804 Anti-Ribeye Antibody, clone 3D12.1

Anti-Ribeye Antibody, clone 3D12.1, Cat. No. MABN804, is a highly specific mouse monoclonal antibody, that targets Ribeye and has been tested in Western Blotting and Immunohistochemistry (Paraffin).

Anti-Ribeye Antibody, clone 3D12.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: CTBP2 UniProt Number: P56545-2
MABN804
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN804
DescriptionAnti-Ribeye Antibody, clone 3D12.1
Alternate Names
  • C-terminal-binding protein 2
  • CtBP2
  • Ribeye
Background InformationHuman C-terminal-binding protein 2 (CtBP2) and Ribeye (UniProt P56545-1 and P56545-2, respectively) are two proteins encoded by the CTBP2 gene (Gene ID 1488) in human, they are produced as a result of alternative splicing. The C-terminal 425-amino acid sequence of Ribeye (aa 561-985) is identical to that of CtBP2 (aa 21-445), however the first 20 amino acids at the CtBP2 N-terminal end is replaced with a 560-amino acid sequence in the case of Ribeye. Ribeye is a protein specific to synaptic ribbons. Ribbon synapses are high-performance synapses built by certain nonspiking sensory neurons, such as photoreceptors, retinal bipolar cells, and inner ear hair cells. The main site of exocytosis of these neurons is characterized by unique presynaptic structures called the synaptic ribbons that associate with large numbers of synaptic vesicles primed for exocytosis and has a direct impact on synaptic signaling. Ribeye is composed of an N-terminal A domain and a C-terminal B domain that is largely identical to CtBP2. The A domain unique to Ribeye has a structural role at the synaptic ribbon, while the B domain of Ribeye possesses lysophosphatidic acid (LPA) acyltransferase (LPAAT) activity that catalyzes the generation of the highly active signaling phospholipid phosphatidic acid (PA) from LPA. LPAAT-deficient Ribeye mutant does not recruit PA-binding proteins to synaptic ribbons, whereas wild-type RIBEYE supports PA-dependent binding, The B domain also contains an NADH-binding subdomain (NBD), which is reported to compete against Arf1 for Arf-GTPase activating protein-3 (ArfGAP3) binding. Ribeye therefore functions as an Arf1 positive regulator at the synaptic ribbon by preventing the inactivation of Arf1 by ArfGAP3.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Ribeye Antibody, clone 3D12.1, Cat. No. MABN804, is a highly specific mouse monoclonal antibody, that targets Ribeye and has been tested in Western Blotting and Immunohistochemistry (Paraffin).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected Ribeye in 10 µg of SH-SY5Y cell lysate.
Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected Ribeye in human cardiac myocytes and human cerebellum tissues.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to the N-terminal region of human Ribeye.
EpitopeN-terminal region
Clone3D12.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificitySpecific for CTBP2 spliced variant Ribeye. Does not react with the canonical CtBP2 isoform.
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CTBP2
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~106 kDa observed (109.2 kDa calculated).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Ribeye in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN804 04055977176988