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MABS2178-100UL Anti-RhoB Antibody, clone 9G5

Anti-RhoB, clone 9G5, Cat. No. MABS2178, is a mouse monoclonal antibody that detects Rho-related GTP-binding protein RhoB and has been tested for use in Flow Cytometry.

MABS2178-100UL
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABS2178-100UL
DescriptionAnti-RhoB Antibody, clone 9G5
Alternate Names
  • Rho-related GTP-binding protein RhoB
  • Rho cDNA clone 6
  • h6
Background InformationRho-related GTP-binding protein RhoB (UniProt: P62745; also known as Rho cDNA clone 6, h6) is encoded by the RHOB (also known as ARH6, ARHB) gene (Gene ID: 388) in human. RhoB is a member of the Ras super-family of small GTP-binding proteins, which is involved in hydrolysis of GTP and is active in GTP-bound form. RhoB is a short-lived Rho GTPase whose expression is inducible by a variety of stimuli including growth factors and stress stimuli. Its levels are up-regulated by DNA damaging agents, such as hydrogen peroxide or ionizing radiation. RhoB plays a negative role in tumorigenesis as its deletion is known to cause tumor formation. It is reported to mediate apoptosis in neoplastically transformed cells following DNA damage. It is not considered to be essential for development, but affects cell adhesion and growth factor signaling in transformed cells. RhoB displays 83% homology with RhoA. However, unlike RhoA, which is cytosolic and translocates to plasma membrane upon activation, RhoB localizes to endosomes/multivesicular bodies. It participates in sorting and degradation of growth factors and cytokine receptors. RhoB has three nucleotide-binding regions (aa 12-19; 59-63; and 117-120). RhoB is shown to be critically required for the inflammatory response of endothelial cells to TNF alpha, possibly through MAP kinase activation downstream of the TNFR.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in PBS without azide.
Applications
ApplicationAnti-RhoB, clone 9G5, Cat. No. MABS2178, is a mouse monoclonal antibody that detects Rho-related GTP-binding protein RhoB and has been tested for use in Flow Cytometry.
Key Applications
  • Flow Cytometry
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal half of human Rho B.
Clone9G5
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 9G5 is a mouse monoclonal antibody that detects RhoB. it targets an epitope within 19 amino acids from the C-terminal half.
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • RHOB
  • ARH6
  • ARHB
Purification MethodProtein G purified
UniProt Number
Molecular Weight22.12 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in HeLa cells.

Flow Cytometry Analysis: 1 µg of this antibody detected RhoB in one million HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS2178-100UL 04061841078210