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NA19L Anti-Replication Protein A (Ab-3) Mouse mAb (RPA34-20)

Anti-Replication Protein A (Ab-3) Mouse mAb (RPA34-20) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-RP-A

NA19L
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, YeastMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the complexed (phosphorylated) and unphosphorylated subunits of replication protein A in HeLa and U293 cells and colon carcinoma tissue.
Catalogue NumberNA19L
Brand Family Calbiochem®
SynonymsAnti-RP-A
Application Data
Detection of human replication protein A by immunocytochemical staining. Sample: HeLa cells fixed with methanol. Primary antibody: Anti-Replication Protein A, Human (Mouse) (Cat. No. NA19L) (1.25 μg/ml). Detection: fluorescence.
References
ReferencesDin, S., et al. 1990. Genes Dev. 4, 968.
Brill, S.J. and Stillman, B. 1989. Nature 342, 92.
Stillman, B., 1989. Annu. Rev. Cell. Biol. 5, 197.
Tsurimoto, T. and Stillman, B. 1989. EMBO J. 8, 3883.
Wobbe, C.R., et al. 1987. Proc. Natl. Acad. Sci. 84, 1834.
Product Information
FormLyophilized
FormulationLyophilized from 20 mM ammonium bicarbonate solution, 100 µg BSA.
Positive controlHeLa or U293 cells or colon carcinoma tissue
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Paraffin Sections
Application NotesImmunoblotting (5 µg/ml)
Immunofluorescence (2.5 µg/ml)
Immunoprecipitation (1 µg/reaction)
Paraffin Sections (2.5 µg/ml, heat pre-treatment required)
Application CommentsReplication protein A (Ab-3) will react with both the complexed (phosphorylated) form of the protein as well as the free (unphosphorylated) 34 kDa subunit. Replication protein A can be used as a marker for cell division. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenreplication protein A purified from U293 cells
ImmunogenHuman
Epitopewithin the p34 subunit of replication protein A
CloneRPA34-20
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
  • Mouse
  • Yeast
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsWe recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
NA19L 0

Documentation

Anti-Replication Protein A (Ab-3) Mouse mAb (RPA34-20) MSDS

Title

Safety Data Sheet (SDS) 

Anti-Replication Protein A (Ab-3) Mouse mAb (RPA34-20) Certificates of Analysis

TitleLot Number
NA19L

References

Reference overview
Din, S., et al. 1990. Genes Dev. 4, 968.
Brill, S.J. and Stillman, B. 1989. Nature 342, 92.
Stillman, B., 1989. Annu. Rev. Cell. Biol. 5, 197.
Tsurimoto, T. and Stillman, B. 1989. EMBO J. 8, 3883.
Wobbe, C.R., et al. 1987. Proc. Natl. Acad. Sci. 84, 1834.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-September-2007 RFH
SynonymsAnti-RP-A
ApplicationImmunoblotting (5 µg/ml)
Immunofluorescence (2.5 µg/ml)
Immunoprecipitation (1 µg/reaction)
Paraffin Sections (2.5 µg/ml, heat pre-treatment required)
Application Data
Detection of human replication protein A by immunocytochemical staining. Sample: HeLa cells fixed with methanol. Primary antibody: Anti-Replication Protein A, Human (Mouse) (Cat. No. NA19L) (1.25 μg/ml). Detection: fluorescence.
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the complexed (phosphorylated) and the ~34 kDa unphosphorylated subunit of replication protein A.
BackgroundThe replication system of a cell contains several essential components among which are replication factor A (RFA, RPA, or HeLa cell SSB), PCNA, and replication factor C (RFC) as well as several other proteins. RPA, a multi-subunit protein containing a 70 kDa, 34 kDa, and 11 kDa protein, may play a critical regulatory role in replication based on its functioning during both the initiation and elongation stages of DNA replication. The protein is highly conserved, having been identified in a range of species from yeast (Saccharomyces cerevisiae) to humans. The primary function of RPA appears to be as an auxiliary protein for both DNA polymerase α and δ. RPA undergoes a cell cycle dependent phosphorylation, which is restricted to the p34 protein. In addition to existing as part of the multi-subunit structure, p34 has also been identified as being present in a free, unphosphorylated form. Recent data shows that RPA is restricted to the nucleus and is overexpressed in transformed cells as well as in several tumor cells.
HostMouse
Immunogen speciesHuman
Immunogenreplication protein A purified from U293 cells
Epitopewithin the p34 subunit of replication protein A
CloneRPA34-20
IsotypeIgG2a
Specieshuman, mouse, yeast
Positive controlHeLa or U293 cells or colon carcinoma tissue
FormLyophilized
FormulationLyophilized from 20 mM ammonium bicarbonate solution, 100 µg BSA.
PreservativeNone
CommentsReplication protein A (Ab-3) will react with both the complexed (phosphorylated) form of the protein as well as the free (unphosphorylated) 34 kDa subunit. Replication protein A can be used as a marker for cell division. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsWe recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h.
Toxicity Standard Handling
ReferencesDin, S., et al. 1990. Genes Dev. 4, 968.
Brill, S.J. and Stillman, B. 1989. Nature 342, 92.
Stillman, B., 1989. Annu. Rev. Cell. Biol. 5, 197.
Tsurimoto, T. and Stillman, B. 1989. EMBO J. 8, 3883.
Wobbe, C.R., et al. 1987. Proc. Natl. Acad. Sci. 84, 1834.