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MABF936 Anti-Regnase-1/Zc3h12a Antibody, clone 15D11

This Anti-Regnase-1/Zc3h12a Antibody, clone 15D11 is validated for use in Western Blotting for the detection of Regnase-1/Zc3h12a.

Anti-Regnase-1/Zc3h12a Antibody, clone 15D11 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: Zc3h12a, Mcpip, Mcpip1 UniProt Number: Q5D1E7
MABF936
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MWBRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF936
DescriptionAnti-Regnase-1/Zc3h12a Antibody, clone 15D11
Alternate Names
  • Ribonuclease ZC3H12A
  • MCP-induced protein 1
  • Regnase-1
  • Zinc finger CCCH domain-containing protein 12A
Background InformationRibonuclease ZC3H12A (UniProt Q5D1E7; also known as MCP-induced protein 1, Regnase-1, Zinc finger CCCH domain-containing protein 12A) is encoded by the Zc3h12a (also known as Mcpip, Mcpip1) gene (Gene ID 230738) in murine species. Regnase-1 is an endonuclease that is involved in post-transcriptional gene regulation by cleaving target mRNAs or the terminal loop of pre-microRNAs. Regnase-1 negatively regulates Il6, Il12b, Il2, Icos, Ox40, Ctla4, cRel mRNAs, as well as its own mRNA (Zc3h12a). In T cells, regnase-1 and an RNA-binding protein roquin act together to inhibit T helper (TH) cell differentiation by negatively regulating mRNAs encoding the TH17 cell-promoting factors IL-6, ICOS, c-Rel, IRF4, IκBNS and IκBζ. This cooperation requires RNA-binding activity of roquin and the endonuclease activity of regnase-1. Upon T-cell receptor (TCR) activation following antigen binding, however, both roquin and regnase-1 are cleaved and inactivated by the paracaspase MALT1, allowing TH17 differentiation to proceed.
References
Product Information
FormatPurified
PresentationPurified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Regnase-1/Zc3h12a Antibody, clone 15D11 is validated for use in Western Blotting for the detection of Regnase-1/Zc3h12a.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected regnase-1 in lysates from wild-type, but not Zc3h12a-/-, mouse embryonic fibroblasts/MEFs (Jeltsch, K.M., et al. (2014). Nat. Immunol. 15(11):1079-1089).
Biological Information
ImmunogenRecombinant full-length mouse regnase-1.
Clone15D11
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityClone 15D11 detected regnase-1 in wild-type, but not Zc3h12a-/-, mouse embryonic fibroblasts/MEFs (Jeltsch, K.M., et al. (2014). Nat. Immunol. 15(11):1079-1089).
IsotypeIgG2aκ
Species Reactivity
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Zc3h12a
  • Mcpip
  • Mcpip1
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~70 kDa observed.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in MEF1 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Regnase-1/Zc3h12a in 10 µg of MEF1 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF936 04055977312904

Documentation

Anti-Regnase-1/Zc3h12a Antibody, clone 15D11 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Regnase-1/Zc3h12a Antibody, clone 15D11 Certificates of Analysis

TitleLot Number
Anti-Regnase-1/Zc3h12a, clone 15D11 - 3164797 3164797
Anti-Regnase-1/Zc3h12a, clone 15D11 - 3378595 3378595
Anti-Regnase-1/Zc3h12a, clone 15D11 - 3808637 3808637
Anti-Regnase-1/Zc3h12a, clone 15D11 - 3942729 3942729
Anti-Regnase-1/Zc3h12a, clone 15D11 - 4187027 4187027
Anti-Regnase-1/Zc3h12a, clone 15D11 -Q2640165 Q2640165