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05-530-I Anti-Rad51 Antibody, clone 3C10

This Anti-Rad51 Antibody, clone 3C10 is validated for use in Western Blotting, Immunoprecipitation for the detection of Rad51 .

Anti-Rad51 Antibody, clone 3C10 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: RAD51, RAD51A, RECA UniProt Number: Q06609
05-530-I
200 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IPMPurifiedMonoclonal Antibody
Description
Catalogue Number05-530-I
Replaces05-530
DescriptionAnti-Rad51 Antibody, clone 3C10
Alternate Names
  • DNA repair protein RAD51 homolog 1
  • HsRAD51
  • hRAD51
  • RAD51 homolog A
  • Rad51
Background InformationDNA repair protein RAD51 homolog 1 (UniProt Q06609; also known as BRCA1/BRCA2-containing complex subunit 5, Homolog of E. coli RecA, hRAD51, HsRAD51, RAD51 homolog A, RecA-like protein, Recombination protein A) is encoded by the RAD51 (also known as HRAD51, HsT16930, BRCC5, MRMV2, RAD51A, RECA) gene (Gene ID 5888) in human. RAD51 is a member of the RecA family recombinases. It interacts with BRCA1 and BRCA2 to activate homologous recombination. RAD51 exhibits DNA-dependent ATPase activity and participates in double-strand break (DSB) repair by unwinding DNA to form a helical nucleoprotein filament. It is highly expressed in the testis and thymus and lower expression levels are found in the small intestine, placenta, colon, pancreas, and ovaries.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Rad51 Antibody, clone 3C10 is validated for use in Western Blotting, Immunoprecipitation for the detection of Rad51 .
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application NotesImmunoprecipitation Analysis: Reported by an independent laboratory to immunoprecipitate.
Biological Information
ImmunogenRecombinant protein corresponding to human Rad51.
Clone3C10
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Mouse, Rat based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • RAD51
  • RAD51A
  • RECA
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~37 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Rad51 in 10 µg of HeLa nuclear extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
05-530-I 04055977173369

Documentation

Anti-Rad51 Antibody, clone 3C10 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Rad51 Antibody, clone 3C10 Certificates of Analysis

TitleLot Number
Anti-Rad51, clone 3C10 (mouse monoclonal) Monoclonal Antibody Q2922327
Anti-Rad51, clone 3C10 - 3436195 3436195
Anti-Rad51, clone 3C10 - 3832627 3832627
Anti-Rad51, clone 3C10 - 3931783 3931783
Anti-Rad51, clone 3C10 - 4137149 4137149
Anti-Rad51, clone 3C10 - 4149123 4149123
Anti-Rad51, clone 3C10 -Q2574784 Q2574784

Technical Info

Title
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.