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MABC1137-100UG Anti-RKIP Antibody, clone 4A11

Detects Raf kinase inhibitor protein (Phosphatidylethanolamine-binding protein 1) using this mouse monoclonal Anti-RKIP, clone 4A11, Cat. No. MABC1137, tested for use in Western Blotting, Immunocytochemistry, and Immunohistochemistry (Paraffin).

Anti-RKIP Antibody, clone 4A11 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: PEBP1, PBP, PEBP UniProt Number: P30086
MABC1137-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICC, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC1137-100UG
DescriptionAnti-RKIP Antibody, clone 4A11
Alternate Names
  • Raf kinase inhibitor protein
  • Phosphatidylethanolamine-binding protein 1
  • PEBP-1
  • HCNPpp
  • Neuropolypeptide h3
  • Prostatic-binding protein
Background InformationPhosphatidylethanolamine-binding protein 1 (UniProt: P30086; also known as PEBP-1, HCNPpp, Neuropolypeptide h3, Prostatic-binding protein, Raf kinase inhibitor protein, RKIP) is encoded by the PEBP1 (also known as PBP, PEBP) gene (Gene ID: 5037) in human. RKIP is as member of the phosphatidylethanolamine-binding-protein (PEBP) family that antagonizes multiple cell-survival pathways, such as the Ras-Raf-1, MEK/ERK, NF- B, and G-protein-coupled receptor Kinase 2 and thereby modulates cell growth, apoptosis, motility, invasion and metastasis. Over-expression of RKIP is shown to inhibit c-Src auto-phosphorylation and activation and IL-6-, JAK1 and 2-, and Raf-mediated STAT3 phosphorylation and activation. RKIP overexpression is shown to sensitize tumor cells to chemotherapeutic drug-induced apoptosis. Hence, it is considered as a suppressor of metastasis and a prognostic marker in several cancers. Clone 4A11 exhibits specific staining in formalin-fixed NIH3T3 cells transfected with GFP-PEBP1 and in some pancreatic cancer cells. (Ref.: Odabaei, G., et al. (2004). Adv. Cancer Res. 91; 169-200; Yousuf, S., et al. (2014). PLoS One 9(3); e92478).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibodyIgG1κ in buffer containing PBS without azide
Applications
ApplicationDetects Raf kinase inhibitor protein (Phosphatidylethanolamine-binding protein 1) using this mouse monoclonal Anti-RKIP, clone 4A11, Cat. No. MABC1137, tested for use in Western Blotting, Immunocytochemistry, and Immunohistochemistry (Paraffin).
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected RKIP in human pancreas and human thyroid tissue sections.

Immunohistochemistry Analysis: A representative lot of this antibody detected RKIP in Immunohistochemistry applications (Wang, X., et. al. (2010). J Immunol Methods. 362(1-2):151-60).

Immunocytochemistry Analysis: A representative lot of this antibody detected RKIP in Immunocytochemistry applications (Wang, X., et. al. (2010). J Immunol Methods. 362(1-2):151-60).
Biological Information
ImmunogenPurified native and denatured Raf kinase inhibitor protein (RKIP).
Clone4A11
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 4A11 specifically detects human Raf kinase inhibitor protein, (RKIP).
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PEBP1
  • PBP
  • PEBP
Purification MethodProtein G purified
UniProt Number
Molecular Weight~23 kDa; 21.06 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected RKIP in HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC1137-100UG 04054839529610