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MABF2802-25UL Anti-RANKL (CD254) Antibody, clone IK22-5

Anti-RANKL (CD254), clone IK22-5, Cat. No. MABF2802, is a rat monoclonal antibody that detects RANKL and is tested for use in Flow Cytometry, Immunofluorescence, Immunohistochemistry, Inhibition Assay, and Neutralizing.

MABF2802-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF2802-25UL
DescriptionAnti-RANKL (CD254) Antibody, clone IK22-5
Alternate Names
  • Tumor necrosis factor ligand superfamily member 11
  • Osteoclast differentiation factor
  • ODF
  • Osteoprotegerin ligand
  • OPGL
  • Receptor activator of nuclear factor kappa-B ligand
  • TNF-related activation-induced cytokine
  • TRANCE
  • CD254ed cytokine
  • TRANCE
  • CD254
Background InformationTumor necrosis factor ligand superfamily member 11 (UniProt: O35235; also known as Osteoclast differentiation factor, ODF, Osteoprotegerin ligand, OPGL, Receptor activator of nuclear factor kappa-B ligand, RANKL, TNF-related activation-induced cytokine, TRANCE, CD254, RANKL) is encoded by the Tnfsf11 (also known as Opgl, Rankl, Trance) gene (Gene ID: 21943) in murine species. RANKL is a single-pass type II membrane protein that has a cytoplasmic domain (aa 1-48), a helical domain (aa 49-69) and an extracellular domain (aa 70-316). High levels of RANKL are detected in the peripheral lymph nodes and lower levels are detected in the spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid. Its levels are shown to be up-regulated by T-cell receptor stimulation. RANKL has been identified to affect the immune system and control bone regeneration and remodeling. It is shown to augment the ability of dendritic cells to stimulate naive T-cell proliferation and is considered as an important regulator of interactions between T-cells and dendritic cells. It is shown to induce osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells. In a murine model of collagen-induced arthritis, clone IK22-5 is shown to reduce bone loss at the site of inflammation. Also, it is reported to reduce joint destruction, but not prevent new bone formation, in an animal model of delayed-type hypersensitivity arthritis. (Ref.: Atkinson, SM., et al. (2016). Arthritis Res.Ther. 18; 28; Kamijo, s., et al. (2006). Biochem. Biophys. Res. Commun. 347(1); 124-132).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2a in PBS without azide.
Quality LevelMQ200
Applications
ApplicationAnti-RANKL (CD254), clone IK22-5, Cat. No. MABF2802, is a rat monoclonal antibody that detects RANKL and is tested for use in Flow Cytometry, Immunofluorescence, Immunohistochemistry, Inhibition Assay, and Neutralizing.
Key Applications
  • Flow Cytometry
  • Immunofluorescence
  • Inhibition
  • Immunohistochemistry
  • Neutralizing
Application NotesInhibition Assay: A representative lot of this RANKL blocking antibody reduced the number of subcapsular sinus macrophages in wild-type mice, inhibited osteoclastogenesis, and inhibited erosive joint destruction. (Camara, A., et. al. (2019). Immunity. 50(6):1467-1481; Kamijo, S., et. al. (2006). Biochem Biophys Res. Commun. 347(1):124-32; Atkinson, S.M., et. al. (2016). Arthritis Res Ther. 18:28; Navet, B., et. al. (2018). Cancer (Basel). 10(11):398; Dougall, W.C., et. al. (2019). Clin Transl Immunology. 8(10):e01081).

Neutralizing: A representative lot of this antibody neutralized RANKL and reduced bone loss in collagen-induced arthritis and eliminated most Peyer s patches microfold cells (PP M cells). (Kamijo, S., et. al. (2006). Biochem Biophys Res Commun. 347(1):124-32; Knoop, K.A., et. al. (2009). J. Immunol. 183(9):5738-47).

Flow Cytometry Analysis: A representative lot detected RANKL (CD254) in Flow Cytometry applications ( Ahern, E., et. al. (2017). Clin Cancer Res. 23(19):5789-5801 ; Kamijo, S., et. al. (2006). Biochem Biophys Res Commun. 347(1):124-32).

Immunohistochemistry Applications: A representative lot detected RANKL (CD254) in Immunohistochemistry applications (Kamijo, S., et. al. (2006). Biochem Biophys Res Commun. 347(1):124-32).

Immunofluorescence Analysis: A representative lot detected RANKL (CD254) in Immunofluorescence applications (Knoop, K.A., et. al. (2009). J Immunol. 183(9):5738-47).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenRecombinant fragment corresponding to the extracellular domain of murine RANKL (CD254).
EpitopeExtracellular domain
CloneIK22-5
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRat
SpecificityClone IK22-5 is a rat monoclonal antibody that detects murine Tumor necrosis factor ligand superfamily member 11 (RANKL/CD254). It targets an epitope within the extracellular domain.
IsotypeIgG2aκ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Tnfsf11
  • Opgl
  • Rankl
  • Trance
Purification MethodProtein G purified
UniProt Number
Molecular Weight35.0 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in L5178Y cells transfected with mTRANCE.

Flow Cytometry Analysis: A 1:250 dilution of this antibody detected RANKL in L5178Y cells transfected with mouse RANKL (mTRANCE).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2802-25UL 04065266553727