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MABE341-AF488 Anti-Puromycin, clone 17H1, Alexa Fluor® 488 Conjugate Antibody

This Anti-Puromycin, clone 17H1 is validated for use in Immunocytochemistry for the detection of Puromycin.

Anti-Puromycin, clone 17H1, Alexa Fluor® 488 Conjugate Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair
MABE341-AF488
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICCRAlexaFluor®488Monoclonal Antibody
Description
Catalogue NumberMABE341-AF488
DescriptionAnti-Puromycin, clone 17H1, Alexa Fluor® 488 Conjugate Antibody
Background InformationPuromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that functions as a protein synthesis inhibitor that blocks translation through premature chain termination in the ribosome. Monoclonal antibodies to puromycin may be used with standard immunochemical methods to directly monitor translation, a method known as surface sensing of translation (SUnSET). Part of the molecule resembles the 3' end of the aminoacylated tRNA, making it useful for protein translation analysis. Puromycin induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.
This product is a conjugated version of Cat. No. MABE341.
References
Product Information
FormatAlexaFluor®488
PresentationPurified rat monoclonal conjugate in buffer containing PBS and 15 mg/ml BSA with 0.1% Sodium Azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Puromycin, clone 17H1 is validated for use in Immunocytochemistry for the detection of Puromycin.
Key Applications
  • Immunocytochemistry
Biological Information
ImmunogenPuromycin-incorporated neosynthesized proteins at multiple molecular weights
Clone17H1
Concentration0.5 mg/mL
HostRat
SpecificityThis antibody detects Puromycin-incorporated neosynthesized proteins.
Species Reactivity
  • Human
Species Reactivity NoteDemonstrated to react with Human test sample, preincubated with Puromycin. Predicted to react with all species when test sample is incubated with Puromycin.
Antibody TypeMonoclonal Antibody
Purification MethodProtein G Purified
Molecular WeightRefer to Cat. No. MABE341 for observed molecular weight information.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in untreated and Puromycin treated HeLa cells.

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Puromycin-incorporated neosynthesized proteins in HeLa cells treated with Puromycin only.

Alexa Fluor® is a registered trademark of Life Technologies®.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE341-AF488 04053252973895

Documentation

Anti-Puromycin, clone 17H1, Alexa Fluor® 488 Conjugate Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-Puromycin, clone 17H1, Alexa Fluor® 488 Conjugate Antibody Certificates of Analysis

TitleLot Number
Anti-Puromycin, clone 17H1, Alexa Fluor® 488 Conjugate - Q2425063 Q2425063
Anti-Puromycin, clone 17H1, Alexa Fluor® 488 Conjugate - 3691372 3691372
Anti-Puromycin, clone 17H1, Alexa Fluor® 488 Conjugate - 3855700 3855700
Anti-Puromycin, clone 17H1, Alexa Fluor® 488 Conjugate - 3991432 3991432
Anti-Puromycin, clone 17H1, Alexa Fluor® 488 Conjugate - 4127491 4127491
Anti-Puromycin, clone 17H1, Alexa Fluor® 488 Conjugate Monoclonal Antibody 3093931
Anti-Puromycin, clone 17H1, Alexa Fluor® 488 Conjugate_2822507 2822507

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies