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MABF973 Anti-Proteinase 3/PR3 Antibody, clone MCPR3-3

Anti-Proteinase 3 Antibody, clone MCPR3-3 is an antibody against PR3 for use in ELISA, Flow Cytometry, and Western Blotting.

Anti-Proteinase 3/PR3 Antibody, clone MCPR3-3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Inflammation & Autoimmune Mechanisms Gene Symbol: PRTN3, CANCA, MBN, NP4 UniProt Number: P24158
MABF973
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ELISA, FCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF973
DescriptionAnti-Proteinase 3/PR3 Antibody, clone MCPR3-3
Alternate Names
  • Myeloblastin
  • AGP7
  • C-ANCA antigen
  • Leukocyte proteinase 3
  • Neutrophil proteinase 4
  • NP-4
  • P29
  • PR-3
  • PR3
  • Wegener autoantigen
Background InformationMyeloblastin (EC 3.4.21.76; UniProt P24158; also known as AGP7, C-ANCA antigen, Leukocyte proteinase 3, Neutrophil proteinase 4, NP-4, P29, PR-3, PR3, Wegener autoantigen) is encoded by the PRTN3 (also known as CANCA, MBN, NP4) gene (Gene ID 5657) in human. Proteinase 3 (PR3) is one of four neutral serine proteases (elastase, cathepsin G, PR3, and neutrophil serine protease 4) stored as fully processed mature enzymes in azurophil granules of human neutrophils. Instead of being targeted to granules after their synthesis, some PR3 molecules end up on the surface of neutrophil plasma membrane in either pro- or mature form. The degree of such surface expression is genetically determined, but the surface exposure and pericellular activity of PR3 around neutrophils can be further upregulated upon neutrophil priming and activation. PR3 autoantibody (anti-neutrophil cytoplasmic antibodies or ANCA) is the main pathogenic feature in patients suffering from granulomatosis with polyangiitis (GPA; formerly called Wegener granulomatosis). ANCAs are shown to activate cytokine-primed neutrophils in vitro by cross-linking surface-exposed PR3 and Fcγ receptors. PR3 activity and/or its inactivation by alpha 1-antitrypsin/alpha-1 proteinase inhibitor (α1PI) varies in the human population and contributes to the risk for GPA manifestations either at onset, during relapses, or during systemic progression. PR3 is initially produced with a signal peptide sequence (a.a. 1-25), an N-terminal and a C-terminal propeptide sequence (a.a. 26-27 and 249-256, respectively), the removal of which yields the mature enzyme (a.a. 28-248).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Proteinase 3 Antibody, clone MCPR3-3 is an antibody against PR3 for use in ELISA, Flow Cytometry, and Western Blotting.
Key Applications
  • Western Blotting
  • ELISA
  • Flow Cytometry
Application NotesWestern Blotting Analysis: 4 µg/mL from a representative lot detected 2-5 µg of purified human neutrophil PR3, as well as 1 µg of both pro- and mature forms of recombinant human PR3 under non-reducing condition (Courtesy of Amber Hummel, Mayo Clinic, Rochester, MN).
Western Blotting Analysis: 2-8 µg/mL from a representative lot detected 5 µg of purified human neutrophil PR3 under non-reducing condition, while greatly reduced reactivity was seen upon sample reduction (Courtesy of Amber Hummel, Mayo Clinic, Rochester, MN).
Flow Cytometry Analysis: A representative lot bound immobilized recombinant human PR3 via a distinct epitope than those recognized by clone MCPR3-2 and MCPR3-7 (Cat. No. MABT340 and MABT403, respectively) as determined by FACS analysis of bead-based competition binding assay (Silva, F., et al. (2010). J. Autoimmun. 35(4) 299-308).
Flow Cytometry Analysis: A representative lot bound recombinant human PR3-, but not murine PR3-, coated Talon-beads as determined by FACS analysis. (Silva, F., et al. (2010). J. Autoimmun. 35(4) 299-308).
ELISA Analysis: A representative lot was immobilized on well surface and employed to capture recombinant human PR3, followed by affinity pull-down of PR3 autoantibodies (anti-neutrophil cytoplasmic antibodies or ANCA) from patients serum samples by the captured PR3 and the subsequent detection of the bound ANCA by alkaline phosphatase-conjugated goat anti-human IgG. (Silva, F., et al. (2010). J. Autoimmun. 35(4) 299-308; Sun, J., et al. (1998). J. Immunol. Methods. 211(1-2):111-123).
Biological Information
ImmunogenPurified human neutrophil PR3.
EpitopeOn the opposite side (the back side) of the substrates- and α1-PI-binding site (the front side) (Silva, F., et al. (2010). J. Autoimmun. 35(4) 299-308).
Clone MCPR3-3
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone MCPR3-3 competed against PR3 activity-neutralizing autoantibodies (neutralizing ANCAs) for PR3 binding. The ratios of OD readings obtained with PR3-complexed clone MCPR3-2 (Cat. No. MABT340) to the corresponding ODs obtained with PR3-complexed clone MCPR3-3 in ELISA-based serum ANCA measurements correlate well with the neutralizing activity of ANCAs in patients serum samples (Silva, F., et al. (2010). J. Autoimmun. 35(4) 299-308).
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PRTN3
  • CANCA
  • MBN
  • NP4
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~29 kDa observed. 27.81 kDa (Pro-PR3; a.a. 1-256), 25.14 kDa (N-terminal signal and propeptide removed; a.a. 28-256), 24.25 kDa (Mature; a.a. 28-248) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceIdentity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be IgG1κ.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF973 04055977333626

Documentation

Anti-Proteinase 3/PR3 Antibody, clone MCPR3-3 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Proteinase 3/PR3 Antibody, clone MCPR3-3 Certificates of Analysis

TitleLot Number
Anti-Proteinase 3/PR3, clone MCPR3-3 3077364
Anti-Proteinase 3/PR3, -Q2665972 Q2665972
Anti-Proteinase 3/PR3, clone MCPR3-3 - 3189717 3189717
Anti-Proteinase 3/PR3, clone MCPR3-3 - 3709407 3709407
Anti-Proteinase 3/PR3, clone MCPR3-3 - 3996348 3996348
Anti-Proteinase 3/PR3, clone MCPR3-3 Monoclonal Antibody 2992640
Anti-Proteinase 3/PR3, clone MCPR3-3 Monoclonal Antibody 3316665