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PC255L Anti-Parvalbumin Rabbit pAb

This Anti-Parvalbumin Rabbit pAb is validated for use in Frozen Sections, Immunofluorescence, Vibrotome Sections for the detection of Parvalbumin.

Anti-Parvalbumin Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
PC255L
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RRbPolyclonal Antibody
Description
OverviewRecognizes the parvalbumin protein in rat muscle, thalamus, hippocampus, and cortex tissue.
Catalogue NumberPC255L
Brand Family Calbiochem®
References
ReferencesHeizmann, C.W. 1993. Acta Neurobiol. Exp. 53, 15-23.
Baimbridge, K.G., et al. 1992. Trends Neurosci. 15, 303.
Heizmann, C.W. and Hunziker, W. 1992. Trends Biochem. Sci. 16, 98.
Product Information
FormLyophilized
FormulationUndiluted serum.
Positive controlRat muscle, rat thalamus, hippocampus or cortex tissue
Preservative≤0.1% sodium azide
Applications
Application ReferencesSpecificity Mithani, S., et al. 1987. Psychopharmacology 93, 94.
Key Applications Frozen Sections
Immunofluorescence
T Cell Co-stimulation
Application NotesFrozen Sections (1:4000-1:8000 for biotin-streptavidin/ peroxidase detection)
Immunofluorescence (1:400-1:800, indirect)
Vibrotome Sections (1:4000-1:8000 for biotin-streptavidin/ peroxidase detection)
Application CommentsAntibody specificity was examined in the rat thalamus, hippocampus, and cortex (see application references). Detection of primary antibody with biotin-streptavidin/peroxidase reagents according to manufacturers directions. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenpurified rat muscle paravalbumin
ImmunogenRat
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Resulting reconstituted solution will contain ≤0.1% sodium azide and 1% BSA. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC255L 0

Documentation

Anti-Parvalbumin Rabbit pAb MSDS

Title

Safety Data Sheet (SDS) 

Anti-Parvalbumin Rabbit pAb Certificates of Analysis

TitleLot Number
PC255L

References

Reference overview
Heizmann, C.W. 1993. Acta Neurobiol. Exp. 53, 15-23.
Baimbridge, K.G., et al. 1992. Trends Neurosci. 15, 303.
Heizmann, C.W. and Hunziker, W. 1992. Trends Biochem. Sci. 16, 98.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision09-October-2007 RFH
ApplicationFrozen Sections (1:4000-1:8000 for biotin-streptavidin/ peroxidase detection)
Immunofluorescence (1:400-1:800, indirect)
Vibrotome Sections (1:4000-1:8000 for biotin-streptavidin/ peroxidase detection)
DescriptionRabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes the parvalbumin protein.
BackgroundSeveral EF-hand calcium-binding proteins have been identified within the nervous system, which includes calbindin, calretinin, and parvalbumin. These cytosolic calcium-binding proteins modulate and mediate the function of the intracellular second messenger calcium. These proteins are believed to function in the buffering, transport and regulation of calcium. Calbindin, calretinin, and parvalbumin are localized in distinct neuronal subpopulations, and antibodies against these proteins are useful markers for neuranatomical and developmental studies.
HostRabbit
Immunogen speciesRat
Immunogenpurified rat muscle paravalbumin
IsotypeIgG
Specieshuman, mouse, rat
Positive controlRat muscle, rat thalamus, hippocampus or cortex tissue
FormLyophilized
FormulationUndiluted serum.
Preservative≤0.1% sodium azide
CommentsAntibody specificity was examined in the rat thalamus, hippocampus, and cortex (see application references). Detection of primary antibody with biotin-streptavidin/peroxidase reagents according to manufacturers directions. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Resulting reconstituted solution will contain ≤0.1% sodium azide and 1% BSA. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
Toxicity Standard Handling
ReferencesHeizmann, C.W. 1993. Acta Neurobiol. Exp. 53, 15-23.
Baimbridge, K.G., et al. 1992. Trends Neurosci. 15, 303.
Heizmann, C.W. and Hunziker, W. 1992. Trends Biochem. Sci. 16, 98.
Application referencesSpecificity Mithani, S., et al. 1987. Psychopharmacology 93, 94.