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PC10L Anti-Pan-Ras Asp¹² (Ab-1) Rabbit pAb

Anti-Pan-Ras Asp¹² (Ab-1) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
PC10L
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RRbPolyclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~21 kDa H-, K-, and N-Ras proteins containing aspartic acid at position 12.
Catalogue NumberPC10L
Brand Family Calbiochem®
References
ReferencesBos, J.L., et al. 1988. Mutation Res. 195, 255.
Furth, M.E., et al. 1987. Oncogene 1, 47.
Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
Taparowsky, E., et al. 1983. Cell 34, 581.
Ellis, R.W., et al. 1981. Nature 292, 506.
Shih, T.Y., et al. 1990 Nature 287, 686.
Product Information
FormLyophilized
FormulationLyophilized from 20 mM ammonium bicarbonate, 100 µg BSA.
Negative controlSW480 cells
Positive controlHS578T cells or purified recombinant K-Ras Asp¹² protein
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1-5 µg/ml)
Application CommentsDoes not react with rasGly-12 or rasVal-12 p21's. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide (KLVVVGADGVGK) corresponding to amino acids 5-16 of Ras (H, K, or N) with Asp at position 12
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage with 0.1% sodium azide. Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC10L 0

Documentation

Anti-Pan-Ras Asp¹² (Ab-1) Rabbit pAb MSDS

Title

Safety Data Sheet (SDS) 

Anti-Pan-Ras Asp¹² (Ab-1) Rabbit pAb Certificates of Analysis

TitleLot Number
PC10L

References

Reference overview
Bos, J.L., et al. 1988. Mutation Res. 195, 255.
Furth, M.E., et al. 1987. Oncogene 1, 47.
Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
Taparowsky, E., et al. 1983. Cell 34, 581.
Ellis, R.W., et al. 1981. Nature 292, 506.
Shih, T.Y., et al. 1990 Nature 287, 686.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-June-2011 RFH
ApplicationImmunoblotting (1-5 µg/ml)
DescriptionPurified rabbit polyclonal antibody. Recognizes the ~21 kDa H-, K-, and N-Ras proteins.
BackgroundThe human ras gene family consists of three identified members H-, K- and N-ras encoding proteins of 188-189 amino acids and 21,000 (p21) molecular weight. Human H-and K-ras are the homologues of v-H- and v-K-ras sequences originally isolated from Harvey and Kirsten strains of rat sarcoma viruses. Normal human cellular ras genes are referred to as proto-oncogenes, and have the potential for activation to oncogenes by mutations occurring in codons 12, 13 and 61; such mutated, activated and transforming ras genes have been identified and isolated from human tumors and cultured tumor cells. Although the expression patterns of ras proto-oncogene proteins in normal human tissues are known, similar information for activated ras oncogene encoded p21's and its relevance to human disease diagnosis and prognosis remains to be determined.
HostRabbit
Immunogena synthetic peptide (KLVVVGADGVGK) corresponding to amino acids 5-16 of Ras (H, K, or N) with Asp at position 12
IsotypeIgG
Specieshuman, mouse, rat
Positive controlHS578T cells or purified recombinant K-Ras Asp¹² protein
Negative controlSW480 cells
FormLyophilized
FormulationLyophilized from 20 mM ammonium bicarbonate, 100 µg BSA.
PreservativeNone
CommentsDoes not react with rasGly-12 or rasVal-12 p21's. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage with 0.1% sodium azide. Avoid freeze/thaw cycles of solutions.
Toxicity Standard Handling
ReferencesBos, J.L., et al. 1988. Mutation Res. 195, 255.
Furth, M.E., et al. 1987. Oncogene 1, 47.
Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
Taparowsky, E., et al. 1983. Cell 34, 581.
Ellis, R.W., et al. 1981. Nature 292, 506.
Shih, T.Y., et al. 1990 Nature 287, 686.